Intervention Of Shenan Extract On Diabetic Nephropathy Mouse Model And The Molecule Mechanism

Objective:To investigate the protective effect of Shen'an Extraction on diabetic nephropathy mouse's kidney and effect on renal morphous. And the effect on renal Col-â…£,Angâ…¡,AT1R,AGTRAP,CTGF,SOD,MDA,AGEs,RAGEs. Method:The diabetic nephropathy mouse models were induced by intraperitoneal injection of streptozotocin(STZ). The mouse were randomly divided into 6 groups: normal control group; diabetic nephropathy model group; Irbesartan group; high-dose of Shenan group; middle-dose of Shenan group; low-dose of Shenan group. Drug intervention term was 4 weeks. The renal pathological morphous was observed by Masson staining and transmission electron microscope. Relative kidney weight, blood glucose level, serum creatinine content, excretion of the 24 hour urine protein were measured. Renal Angâ…¡content was measured by radioimmunity. The expression of AT1R,AGTRAP,CTGF,RAGE mRNA in renal cortex was detected by Realtime-PCR. The expression of AT1R,AGTRAP,CTGF,RAGE in renal cortex was measured by immunohistochemistry. SOD activity of nephridial tissue was measured by xanthine oxidase method. MDA content of nephridial tissue was measured by thiobarbituric acid reactive substance assay. AGEs content of nephridial tissue was measured by absorption spectrometry.Result:Autonomic activities of diabetic nephropathy mouse reduced notably. Urinary volume of diabetic nephropathy mouse obviously increased.diabetic nephropathy mouse' glomcrulus got hypertrophia and fibrous degeneration. When observed by transmission electron microscope the glomerular basement membrane of diabetic nephropathy mouse became thick, the podocytes swelled, part of foot process coalesced or overgrow. Relative kidney weight, blood glucose level, serum creatinine,excretion of the 24 hour urine protein, Angâ…¡MDA,AGEs content and expression of CTGF,RAGEs,Col-â…£in cortex renis increased obviously (P<0.05 or P<0.01). SOD activity in nephridial tissue. and expression of AT1R,AGTRAP in cortex renis decreased notably (P<0.01). After Intervention of drugs Angâ…¡,MDA,AGEs content and expression of CTGF,RAGEs,Col-â…£in cortex renis decreased obviously (P<0.05 or P<0.01).. SOD activity in nephridial tissue.and expression of AT1R,AGTRAP in cortex renis increased markly (P<0.05 or P<0.01). Conclusion: Shen'an Extraction could protect kidney of diabetic nephropathy mouse. The mechanism was related to its inhibiting effect on CTGF,RAGEs,Col-â…£expression in renal cortex and decreasing the Angâ…¡,MDA,AGEs content in nephridial tissue and promoting effect on AT1R,AGTRAP expression and elevating the SOD activity in nephridial tissue of diabetic nephropathy mouse.Subject words Diabetic nephropathy/TCM therapy; Diabetic nephropathy/empirical study; Shen'an Extraction; Renin-angiotensin system; Oxidative stress;Advanced glycosylation end prodrcts...