Sugar Kidney Huayu Decoction On Early Diabetic Nephropathy Of Non-enzymatic Glycosylation Of Experimental Research

ObjectiveThrough observing the ei'fect of the TCM decoction TangShenHuaYuJian(TSHYJ) on the kidney weight, index of kidney hypertrophy(kidney weight/body weight), blood glucose , blood urea nitrogen(BUN), creatinine(Cr), blood advanced glycation end-products(AGEs), advanced glycation end-products(AGEs) in kidney tissue in the earlier stage of diabetic nephropalhy rats induced by injecting 3 -cytotoxins into abdomen, to explore the relationship between nonenzymatic glycosylation and diabetic nephropathy, and provide an experimental basis for clinical application and for further studying TSHYJ.MethodsThe sixty studied Wistar rats were randomly divided into five groups: normal control group(normal group), model group, high dose of TSHYJ group(high dose group), moderate dose of TSHYJ group(moderate dose group), low dose of TSHYJ group(low dose group). After inducing diabetic models by injecting (3 -cytotoxins into their abdomen successfully, the docoction of TSHYJ was given to the three treated groups and the same dose of saline was given to the normal group and the model group by stomach rearing once per day for six weeks. At the end of six weeks, all of the rats were killed and the kidney weight, index of kidney hypertrophy (kidney weight/body weight), blood glucose, blood urea nitrogen(BUN), creatinine (Cr), blood advanced glycation end-products(AGEs), advanced glycation end-products(AGEs) in kidney tissue of the rats of each group were tested. The results were analyzed with SPSS.Results1 Kidney weight and index of kidney hypertrophy (1) Kidney weight: the kidney weight of model high and low dose groups was distinctly heavier than that of normal group, P<0.01; moderate dose group had no change, P>0.05. (2)Index of kidney hypertrophy: the index of kidney hypertrophy of the three treated and model groups was distinctly higher, P< 0.05 or P <0.01 ; TSHYJ could markedly decrease the index of kidney hypertrophy , compared with the model group, P< 0.05 or P <0.01.2 Blood glucose The blood glucose of the treated and the model groups at pretreatment and post-treatment distinctly increased , compared with the normal group, P <0.01; After treatment, the blood glucose of the treated groups distinctly decreased , compared with the model group P< 0.05 or P <0.01 ; Compared pretreatment with post-treatment, the blood glucose of the model group distinctly increased , P <0.05, the moderate and high dose groups distinctly decreased , P <0.01 , the low dose group had no markedly change.3 BUN and Cr in serum (1)BUN: there were no distinct differences (P > 0.05)among the treated, the mode! and the normal groups. (2)Cr: the serum Cr of thetreated groups distinctly decreased, compared with the model group, P< 0.05 or P <0.01.4 AGEs in serum and AGEs fluorcscene exposure time in kidney tissue (1)AGEs in serum: AGEs in serum of the treated and the model groups distinctly increased , compared with the normal group, P <0.01; AGEs in serum of the treated groups distinctly decreased , compared with the model group, P< 0.05 or P <0.01 ; (2)AGEs fluorescene exposure time in kidney tissue: the exposure time of the treated and the model groups was distinctly shorten, compared with the normal group P <0.01; Compared with the model group, the exposure time of the treated groups was distinctly prolonged, P <0.01.ConclutionTSHYJ has certain renal protective, probably through lowering blood glucose, AGEs in serum and AGEs in kidney tissue, preventing early diabetic nethropathy by hindering and inhibiting nonenzymatie glycosylation . Decreasing the index of kidney hypertrophy and Cr in serum to improve renal function. So TSHYJ is worth further researching and developing.