The Effect Of Exercise And EGCG,L-carnitine Administration On The Weight Loss,Lipid Metabolism And Gene Expression In Obese Rats

Objective:Study the effect of exercise and EGCG, L-carnitine on the weight loss, lipid metabolism and gene expression in obese rats, and research the mechanism of obese rats.Methods:Establish high-fat-diet obesity animal model in4-weeks-old male Sprague-Dawley rats. Study the effect of high-fat diet on the rats'weight, visceral fat coefficient, serum TG, TC, HDL level, the organization triglyceride level, the liver PPARα, PGC-1α, AMPKα2, CPT1, MCAD expression level. Then the50successful modeling rats were randomly divided into the obese control group(group F),the pure exercise intervention group(group S), the exercise+EGCG intervention group(group S+E), the exercise+L-carnitine intervention group(group S+L), the exercise+EGCG+L-carnitine intervention group(group S+E+L). In addition to the group F, the other four groups of rats accept low-intensity aerobic treadmill training. After7weeks, we compared the body weight, visceral fat coefficient, serum TG, TC, HDL level, organization trigiyceride level, the liver PPARa mRNA, PGC-1α mRNA, AMPK α2mRNA, CPTlmRNA, MCADmRNA and the PPAR a, PGC-1α, AMPK a2, CPT1, MCAD protein level of the five groups.Results:(1)The serum TG, TC level of group F was significantly higher than the normal control group(group C), the HDL level of group F was significantly lower than group C(P<0.05).The liver triglyceride level in group F was higher than group C(P<0.05).The PPARα mRNA and protein expression, the PGC-1a mRNA, the AMPK a2protein expression of the group F were significantly lower than group C(P<0.05)(2)Compared with group F, the weight and the serum TG level of group S were significantly lower than group F(P<0.05). The weight and the serum TG level of group S+E, S+L, S+E+L were significantly lower than group F and S(P<0.05). The serum TC level and the liver TG levels of group S+L, S+E+L were significantly lower than group F(P<0.05). The visceral fat coefficient of group S+E+L was significantly lower than group F, the serum HDL level of group S+E+L was significantly higher than group F(P<0.05)(3)Compared with group F, there is no significant difference of the PPAR α,the PGC-1α,the AMPK a2, the CPT1, the MCAD mRNA and protein expression in group S. The PPARα, the PGC-1α, the AMPKα2, the CPTland the MCAD mRNA/protein expression of S+E, S+L, S+E+L were significantly higer than group F. The CPT1mRNA of group S+E, S+L, S+E+L were higher than group S. The PPAR a, the PGC-1a, the AMPK a2, the MCAD mRNA of group S+E+L were also higher than group S+E/S+L or both.Conclusion:(1) Expression of AMPKa2decreased in obese rats, and it down-regulated PGC-1α expression, PPARα expression also decreased, while PPARα mediated downstream target gene-CPTl transcription and translation, CPT1expression declined.(2) Exercise can lose weight. The combination of EGCG or L-carnitine or both may enhance the role of exercise to lose weight. Speeding up the oxidation of fatty acid and altering the lipid metabolism related factors such as PPARα, PGC-1α, AMPKα2, CPT1.MCAD may be one of the possible mechanisms.