| The design and construction of distinctive micro-/nanostructure interfaces basedon various polymers, which can supplement microscopic performances and broadenmore application of macroscopic materials, is of great significance and bright future.With the increasing development of micro-/nano interface processing technology, todesign the micro-/nanotopographical interfaces according to function oriented route,becomes the main trend of development. Especially to the development of somemicroscaled smart device, tissue engineering scaffolds and biointerface, thepersonalized design route is very necessary. As the aging population is increasing inChina, the public healthcare is attracting much more attention, and meanwhile thebiointerface research becomes a focus. It is important to explore the interfacialinteraction between biosystem such as cells, biomolecules, tissue and biomaterials, inthe application of drug delivery, tissue implant and transplantable biodevice. As weknow, most biomolecules have1-100nm size, and the sub-cellular is below1μm,exploring the interaction in microscale may gain more novel function and potentialapplications.In this thesis, by utilizing the basic micro-/nano interface processing technology,we fabricated novel functional smart materials and biointerfacial materials, andfurther investigated the interaction mechanism between micro-/nanotopographies andbiological systems. The main contents of this thesis include the following three parts:1. Designing micro-/nanointerface based on polyaniline and demonstrating theirpotential application. Polyaniline, as one of high performance conducting polymers has various properties such as electroactivity, reversible doping chemistry, smartresponsibility, and tunable nanotopography. It was widely used in fields of smartsensor, electrostatic shielding device, superhydrophobic interface, biomaterials, etc.Based on the reversible doping of polyaniline, we fabricated a kind ofhigh-performance polyaniline/polyvinyl alcohol nanocomposite actuators with tunablestimuli-responsive properties, and revealed the smart actuating performance. Further,we constructed various polyaniline nanostructure interfaces, combining basicmicro-processing method and chemical/electrochemical polymerization of polyaniline.We also revealed the prospects of their application in smart gas sensor, micro-faradaycage and microreactor.2. Bone-friendly poly(etheretherketone) based polyaniline nano-biointerface tomediate controlled behaviors of mesenchymal stem cells (MSCs). We integrated thesmart PANI nano-biointerface and the high-performance specific engineering polymerPEEK through a―bottom-up‖route. Greatly enhanced MSC performance such as fastadhesion, fast spreading-out and tunable migration on this bone-similar substrate wereobtained. We qualitatively-quantitatively analyzed the chemical factor/nanocuecoeffect of substrates on cell controlled behaviors. Specially, we took the PANInanofiber array interface as a typical model, and further refined a characteristicparameter relatively effective area (EA) to describe the nanotopographical factor oncell behaviors, which have a universal applicability to most uniform nanointerface. Itis anticipated the―bottom-up‖method allows three dimensional modification ofnanobiointerface on various shapes and complex surfaces of PEEK graft such as flator porous surfaces, which can be used to repair massive bone defects. We believe thiswork would also provide fundamental but crucial information for stem cell study andtissue transplant technologies, which contributes to further applications in stemcell-based therapy and regenerative medicine.3. Gradient polyethylene terephthalate (PET) nanopost array to mediatecontrolled behaviors of cerebral microvascular endothelial cells. We constructed gradient PET nanopost arrays based on two-dimensional colloidal crystal templatemethod and reactive ion etching technology. The gradient PET nanopost array haslinear trend of size change in one-dimension. The cerebral microvascular endothelialcells revealed directional distribution with the cellular long axis parallel to thegradient direction. Moreover, cells on the gradient array show remarkable directionalmigration, which has a bright application future in artificial blood vessel innerinterface, tissue engineering and related biomaterials. |
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