Antioxidant Activity,Antiproliferation Activity And The Mechanism Of Antiproliferation Of Phenolics From Adinandra Leaf

Phytochemicals is interpreted as non-nutrient bioactive plant ingredient in vegetables, fruits, and other plant foods which have been associated to reducing the risk of chronic deseases. Phenolics is a kind of the most important phytochemicals, has cooperative and superimposed effects on antioxidant activity, anti-inflammatory, hypoglycemic, coordinating gene expression in cell proliferation and apoptosis, and balancing physiological metabolism, and they may play a direct role in anticancer activities. Consumption of plant food could reduce probability of chronic diseases, such as diabetes, high blood pressure, cancer, etc. It is increasing attention of nutrition and health, diet is one of the effective ways to prevent and treat disease.Adinandra widely distributed in China, possesses of a variety of biological activities and is of great prospect for research and development. It is rare about the health benefits of Adinandra. In the paper, ramie leaves, good sources for dietary and medical use, are selected to set up evaluation system of phytochemical profiles and bioactivity for analyzing the relationship between phytochemicals profiles and human health. Then, compare the phytochemicals profiles, antioxidant activity and anticancer activity; following, study on the effect of stir fry on the phytochemicals profiles and bioactivity; the contribution of five flavonoids compounds to Adinandra nitida tea; the changes of phytochemicals profiles and bioactivity of Adinandra nitida tea under gastrointestinal digestion environment and the mechanism of anticancer for colon cancer. It is help to elucidate the health benefit of phytochemicals from Adinandra tea and provide the theory and method on the depth of its development. The results are as follows:The models effectively evaluate the relation between the phytochemicals properties from different varieties and bioactivities.Adinandra teas were rich in phenolics and flavonoids, their antioxidant capacities were high as revealed by ORAC, PSC and CAA assay. Obvious antiproliferation effect was observed in Hep G2 and MCF-7 cells. Among the four varieties compared, Nitida and Millettii had higher CAA value and anti-proliferation activity, while Latifolia contained considerable bound phenolics.About 5.66 times increase in total water-soluble solid, 34.34 times in phenolics, and 15.27 times increase in flavonoids were calculated in stir fried samples compare to non-stir fried samples. Flavor components were released after stir frying. PSC value was increased 333.05 times, and ORAC values by 13.12 times in stir fried tea samples. Similarly, stir fried tea samples exhibited anti-proliferation activity, whereas it was lacking in non-stir fried leaf. Phenolics from stir fried tea affected Hep G2 cell cycle, induced cell to apoptosis.Composite action between the components in Adinandra tea was analyzed by PSC, ORAC and CAA. In thermal processed tea, EC has the highest PSC value, ORAC value and CAA value in thermal processed tea and non-thermal leaves; howvere, EGC had the strong antiproliferation on Hep G2 cells.After gastrointestinal digestion, phenolics and flavonos contents were increased, flavonoid and anthocyanin contents were decreased. PSC value was decreased, and ORAC value and CAA value were increased. Similarly, section of intestinal fluid exhibited anti-proliferation activity on Caco-2 cells. Phenolics from gastrointestinal digestion blocked the cell cycle and affected Caco-2 Caspase3/9, induced cells to apoptosis.