Follistain Effect The Development Of Bovine Embryo

It is very smooth that bovine embryo technology in vitro, however that is still very poorer than in vivo blasto. The low development rate is still a major bottleneck limit embryo engineering technology. So the study tries to add follistatin increase blastocysts rate.This study used in vitro embryonic operation method, microinjection method, the method of molecular biology, fluorescence immunological method technology etc. The discovery of surprise, there are effects of follistatin supplementation on early cleavage embryo during IVC1and IVC2stag, that can significant improve blastocyst rate at d7, reach to65%.And get the following conclusion.1) there are different effect of follistatin dose on embryo development during IVC1stage. But even if silence the FST gene also won’t affect early cleavage rate, This suggests us may be in the early embryo development stage, especially early cleavage rate stage, follistatin is not necessary, But if add exogenous follistatin can improve the early cleavage rate.2) During IVC1and IVC2stage supplement10ng/mL follistatin can improve blastocyst rate after IVF d7.At IVC1stage use follistatin get more blastocysts than IVC2stage, Add follistatin affect blastocyst rate by improve number of TE cells, but not influence ICM.3) Follistatin supplementation on early cleavage embryo can improve blastocyst rate after IVF d7. Early cleavage embryo can get50%blastocyst rate even without add follistatin that is more significant than contrast. Without supplement follistatin on middle cleavage embryo got lower blastocyst rate than use, that is not more significant than contrast.When use lOng/mL and more noggin, with concentration up. early cleavage rate, blastocyst rate significent decrease, and ICM number cut down.