| This research used self-made growth box at plastic big shed, treated with rich CO2content and regula(?)d air temperature and humidity at9:30-16:30everyday. Through test1,2and3, we study the effect of air temperatures and humidity regulation on graft cucumber nutrition grovth, procreation growth, photosynthesis and stress physiology, finally obtain the following basic conclusion:(1) With the cond(?)ion of CO2content was1243.3μl/L～1300.6μl/L, air temperature was32.7℃～34.1℃(sub-high temperature), the height, stem diameter, leaves number, leaves area, leaves thickness, dry matter accumulation and strong seedling index of cucumber were all increased as treatment period extension and air humidity raised at9:30～16:30everyday. The dry matter distribution in root、stem. flower and fruit of cucumber were increased, but in leaves were reduced as treatment period went by.(2) With the condition of CO2content was1274.9μl/L～1400.4μl/L, air temperature was42.6℃～43.4℃(high temperature), the height, stem diameter, leaves number, leaves area, leaves thickness, dry matter accumulation and strong seedling index of cucumber were all increased as treatment period extension and air humidity raised at9:30-16:30everyday. The dry matter distribution in stem, flower and fruit of cucumber were increased, but in root and leaves were reduced as treatment period went by.(3) With the condition of CO2content was1246.6μl/L～1333.4μl/L, air humidity was94.1%～96.4%(high humidity), the height、stem diameter, leaves number、leaves area, leaves thickness, dry matter accumulation and strong seedling index of cucumber were all increased as treatment period extension and air temperature raised at9:30～16:30everyday. The dry matter distribution in stem、flower and fruit of cucumber were increased, but in root and leaves were reduced as treatment period went by.(4) With the condition of CO2content was1243.3μl/L～1300.6μl/L, air temperature was32.7℃～34.1℃(sub-high temperature), the cucumber female flower number, male flower number, female flower number/male flower number have no obviously difference, fruit、commercial cucumber number、single fruit weight, individual yield, district yield and equivalent yield were all increased, but withered and abnormal fruit rate were reduced with humidity increased at9:30～16:30everyday. (5) With the condition of CO2content was1274.9μl/L～1400.4μl/L, air temperature was42.6℃～43.4℃(high temperature), the cucumber female flower number、male flower number、female flower number/male flower number have no obviously difference, fruit、commercial cucumber number、single fruit weight、individual yield、district yield and equivalent yield were all increased, but withered and abnormal fruit rate were reduced with humidity increased at9:30～16:30everyday.(6) With the condition of CO2content was1246.6μl/L～1333.4μl/L, air humidity was94.1%～96.4%(high humidity), the cucumber female flower number male flower number、female flower number/male flower number have no obviously difference, fruit and commercial cucumber number were increased, withered and abnormal fruit rate were reduced, and single fruit weight、individual yield、district yield and equivalent yield have no difference with temperature increased at9:30-16:30everyday, but single fruit weight、individual yield、district yield and equivalent yield in high temperature condition (42.6℃, T1) rise about1.00%、12.04%.11.92%and11.93%respectively than room temperature.(7) With the condition of CO2content was1243.3μl/L～1300.6μl/L, air temperature was32.7℃～34.1℃(sub-high temperature), the daily variation of net photosynthesis rate around the test were all bimodal curve,-the peak was at11:00andl5:00, the lowest value was at13:00; as humidity raised, net photosynthesis rate increased. The Pn value was21.13μmol·m-2·s-1at high humidity(96.4%, L1),and was increased About64.18%and97.85%than middle humidity(75.2%, L2) and low humidity(56.8%, L3) respectively. Although Pn was cut down about28.32%at11:00, plant could keep higher photosynthesis ability, relieve or eliminate the "midday rest" of photosynthesis. Pn descent, L, was no stoma factor, and L2、L3were stoma factor.The daily variation of transpiration rate in L1、L2treatment was unimodal curve, the peak was both at13:00. in L3, transpiration rate was bimodal curve, the peak was at11:00and15:00. Tr showed L1>L2>L3.As treatment elongation, the content of chlorophyl was low first and increase latter. The content of chlorophyl also increased as humidity rose.The daily variation of starch content among three treatment cucumber leaf was increased first and dropped later, they were showed as L1<L2<L3.(8) With the condition of CO2content was1274.9μl/L～1400.4μl/L, air temperature was42.6℃～43.4℃(high temperature), the daily variation of net photosynthesis rate around the test were all bimodal curve, the peak was at11:00and15:00, the lowest value was at13:00; as humidity raised, net photosynthesis rate increased. The Pn value was22.65μmol·m-2·s-1at high humidity(96.4%, L1),and was increased About87.81%and171.58%than middle humidity (75.2%, L2) and low humidity (56.8%, L3) respectively. Although Pn was cut down about29.46%at11:00, plant could keep higher photosynthesis ability, relieve or eliminate the "midday rest" of photosynthesis. Pn descent, L1、 L2were no stoma factor, and L3was stoma factor. The daily variation of transpiration rate in L1treatment was unimodal curve, the peak was at13:00. in L2,L3, transpiration rate was both bimodal curve, the peak was at11:00and15:00. Tr showed L1>L2>L3.As treatment elongation, the content of chlorophyl was dropped first and increased latter. The content of chlorophyl also increased as humidity rose.The daily variation of starch content among three treatment cucumber leaf was increased first and dropped later, they were showed as L1<L2<L3.(9) With the condition of CO2content was1246.6μl/L-1333.4ul/L, air humidity was94.1%v96.4%(high humidity), the daily variation of net photosynthesis rate around the test were all bimodal curve, the peak was at11:00and15:00, the lowest value was at13:00; the max net photosynthesis rate in T1, T2and T3treatment were32.11μmol·m-2·s-1,29.48μmol·m-2·s-1and27.00μmol·m-2·s-1respectively, the min net photosynthesis rate were22.65μmol·m-2·s-1,21.13μmol·m-2·s-1and20.17μmol·m-2·s-1, all have higher photosynthesis ability. Pn descent, T, was no stoma factor, and T2, T3were stoma factor.The daily variation of transpiration rate in three treatment was unimodal curve, the peak was all at13:00, the value were37.19mmol·m-2·s-1,35.20mmol·m-2·s-1and34.50mmol·m-2·s-1respectively, and have no difference.As treatment elongation, the content of chlorophyl was dropped first and increased latter. The content of chlorophyl also increased as temperature rose, but has no difference.The daily variation of starch content among three treatment cucumber leaf was increased first and dropped later, they were showed as T1<T2<T3, but have no difference.(10) With the condition of CO2content was1243.3μl/L～1400.4μl/L, three experiments show that the osmoregulation substance、Malondialdehyde content、 membrane permeability and protective enzyme activity were all dropped as air humidity increased, its indicated that by self-adaptation and adjusting process, plants could enrich their ability to resist with high temperature threaten, and cut down the level of versity threaten, relieve the injury from stress condition.In all, at the complex action of high CO2(1000～1500μl/L) content、high humidity (95%±5%) and high temperature (40℃～45℃), the height、stem diameter, leaves number, leaves area, leaves thickness and dry matter accumulation of graft cucumber were increased, Elevate plant strong seedling index, fruit growth speed, production and Photosynthesis, meanwhile reduce the withered and abnormal fruit rate, relieve or eliminate the "midday rest" of photosynthesis, enrich plants ability to resist with high temperature threaten, and cut down the level of versity threaten, relieve the injury from stress condition. |
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