Overexpression Of OCT-4/SOX-2Genes In Bone Marrow—Mesenchymal And Umbilical Mesenchymal—Stem Cells Of Wuzhishan Mini Pig

The transcription factors Oct-4and Sox-2have been shown to be critical for maintaining pluripotency and self-renewal of embryonic stem (ES) cells. Oct-4can form a heterodimer with Sox-2to orchestrate an embryonic stem (ES) cell-specific gene expression program. Early embryonic development and ES cells depend on fundamental transcription frameworks that are governed by a common set of "core" stem cell-specific transcription factors, namely Oct-4and Sox-2.FoxD3, a member of the forkhead family of transcriptional regulators, as Oct-4and Sox-2down stream of the target gene, is also required for maintenance of embryonic cells. Identification of key regulatory these genes is an important step in exploiting the mechanisms for embryonic stem cells, embryonic development and cell therapy.In this study, we constructed lentivirus-mediated overexpression vector of Oct-4and Sox-2, and transfected in porcine bone marrow mesenchymal and umbilical mesenchymal stem cells to detect differential genes. Results indicated that Oct-4and Sox-2genes associated with a varied set of genes including regulators of gene expression and modulators of cell function in bone marrow mesenchymal and umbilical mesenchymal stem cells.In this study, we cloned the cDNA sequences of porcine FoxD3gene, identified its expression pattern and functions, to provide new insights for mechanisms of muscle development and identification of stem cells.