The Foundational Studies Of Toxicity Substances In Exotic Invasive Plant Eupatorium Adenophorum Spreng

The aims of the studies are to separate the main toxicity compounents, to establish the quality control standard of raw material and Eupatorium adenophorum Spreng products, and to study the hepatotoxicity and immunotoxicity in vivo.Major achievements of this research are as follows:1. The main toxicity compounds were screened and identified as sesquiterpenoids of leaves of Eupatorium adenophorum Spreng. Results of the acute toxicity in mice showed that the target organs of the toxicity were liver, gall bladder, thymus and spleen.2. Four toxicity sesquterpenoids named9-oxo-10,11-dehydro-agerophorone (Z9),10Hα-9-oxo-agerophorone (Z8),10Hβ-9-oxo-agerophorone (Z7), and2-deoxo-2-(acetyloxy)-9-oxoageraphorone (Z6) were isolated from the dried leaves of Eupatorium adenophorum Spreng.3. Serum levels of AST、ALT、ALP and TBIL、DBI、IBIL content and the pathological changes of hepatic tissue were detected. The results showed that Z9、Z7or Z6could induce mice hepatic injury.4. Indices of thymus and spleen, multiplication of spleen lymphocyte (MTT assay), the NK activity effect, and apoptosis rate of spleen lymphocyte were detected, respectively.The results of our experiments indicated that Z6has the inhibiting effect on immune function of mice.5. We developed an HPLC method for determining three toxicity sesquterpenoids from E.adenophorum. The distribution and dynamic variation in accumulation of the toxicity components in Eupatorium adenophorum were detected. The established HPLC method could be used for component determination in E. adenophorum products for quality control and safety evaluation.