Preparation Of Xiaoqinglong Granule And The Therapeutic Effect Of Chicken Infectious Bronchitis

Xiaoqinglong granule is made from ephedra, White Paeony Root, dried ginger, radixglycyrrhizae preparata, cassia twig, asarum, shizandra and rhizoma pinelliae preparatum, and thenprepared into granules by modern technology; Xiaoqinglong granule is used to treat cough andgasp and the sputum dilute; Pathological changes in lung and spleen. This is a commonprescription for warm cold retained fluid, so known as the ages square.This study optimized the extraction process and quality standards of Xiaoqinglong granule.To provide adequate references for further clinical application.,we carried research on the stability,pharmacology and toxicology.Objective: Determine the optimal extraction process;Improve quality standards; Observedthe stability of agents; Determine the toxicity of Xiaoqinglong granule; Investigate theanti-inflammatory effect and clinical curative effect of granule.Method: With ephedrine hydrochloride and paeoniflorin content as control target, ourselected the best extraction process through orthogonal test; TCL was adopted to analyze iephedra,white paeony root, honey-fried licorice root, shizandra and dried ginger, HPLC was used todetermine content of ephedrine hydrochloride and paeoniflorin; Investigate the stability ofXiaoqinglong granule through acceleration test, accelerated testing light and long-term test;Investigation into the toxicity of granule through the acute toxicity test in mice and chronictoxicity test in rat; The experiment proved that the particles has good anti-inflammatory effects invitro and in vivo. We chose clinical disease of poultry and divided into6groups randomly, andevaluate the therapeutic effect of Xiaoqinglong particle through clinical symptoms andpathological changes.Result:Technical study: The orthogonal experiment results show that,the number ofextraction had significant influence on the content of paeoniflorin and ephedrine hydrochloride; the best fit is A3B1C3, meaning that use12times water soak the medicinal materials, decoctionfor3times, each time1hour.Quality standards: TCL was adopted to analyze iephedra, white paeony root, honey-friedlicorice root, shizandra and dried ginger. The identification method we established is specificity.In the identification test there’s the same colour speck in the preparation Chromatography.Background is clear, good separating degree, nil interference, the result is easy to judge, all theseconform to thin layer chromatography. HPLC was used to determine content of ephedrinehydrochloride and paeoniflorin. Results showed that the linear relation of ephedrine hydrochloridewas good within0.06~1.5μg. The sample solution stable within6h; The linear relation ofephedrine hydrochloride was good within0.047~1.175μg; Mean recovery is104.55%. The samplesolution stability within6h.Stability test: The results of the stability test show that: Xiaoqinglong granule is not stableunder the condition of light and heat, it can be stored at room temperature for18months.Pharma-toxicology: Xiaoqinglong granule is low toxicity, used for a long time without sideeffects, so can be applied to clinical. The granule has anti-inflammatory effects on RAW264.7cells in vitro. Clinical trials show that the total effective rate of Xiaoqinglong granule in high andmedium dose group were more than80%, there is no significant difference with Shuanghuanglianoral liquid.In conclusion, the technology of extracting Xiaoqinglong granule is stable, quality can becontrol, low toxicity, good anti-inflammatory and clinical effec, store at room temperature for18month.