| Schisandra chinensis(Turcz.)Baill has variety of nutrients. It is a good development prospects plant with the use of drug and eating. Lignan which is the main active ingredient of Schisandra chinensis has a high content ofγ-schizandrin. The main functional component isγ-schizandrin. Modern research has shown thatγ-schizandrin has cancer-fighting doxorubicin and scavenging free radicals role. The principal active ingredient are lignan and polysacch-aride. Liposomes as a drug of new agents that can improve drug targeting and reduce drug toxicity.This subject took Schisandra chinensis which was collected in Liao Ning province as material, studied the extract method ofγ-schizandrin by using orthogonal test design, macroporous resin were employed forγ-schizandrin separation and purification. The antioxidant and antimicrobial activities of the extract were also investigated. This study investigated the preparation of theγ-schizandrin liposomes and theγ-schizandrin-doxorubicin liposomes. Research the Physical and Chemical Properties ofγ-schizandrin liposomes, the Physical and Chemical Properties ofγ-schizandrin-doxorubicin liposomes, the drug kinetics. Preliminary study the anti-tumor effect ofγ-schizandrin. The main results were shown as follows:1.The optimum technological conditions of solvent extraction:solvent strength 90%, Liquid:Materal 7:1, processing time 2.5h, extract 3 times; The optimum technological conditions of supercritical CO2 extraction:pressure 30Mpa, temperature 40℃, granularity 40 mesh, time 2h; The optimum extraction conditions of microwave assisted extraction were as follows:power 500 W, solvent-material ratio 10:1, extraction time 8min; The optimum extraction conditions of ultrasonic waves:power180 W, solvent-material ratio 10:1, extraction time 40min.2.AB-8 resin is suitable forγ-schizandrin separation and purification with better capabilities of absorption and desorption in the research. The technological parameters of AB-8 edulcorateγ-schizandrin:concentration ofγ-schizandrin 0.09mg·mL-1; absorption flow rate 1mL·min-1; absorption time 150min; elution rate of 0.5 mL·min-1.3.Adopts rotating evaporation film for offering legal liposomes. Edible with encasulation efficiency as evaluation indexes. By single factor and orthogonal test, for optimum technological conditions 1:0.1,γ-schizandrin grain cindoruk 1%, Rotating evaporation temperature for 60℃, pH 6.9, encapsulation rates 92.4%.4. Prepared by rotating evaporation film legalγ-schizandrin -doxorubicin liposomes. The best preparation process for:Soybean lecithin and cholesterol than for the 1:0.05, Soybean lecithin and than for the adriamycin 2:0.01. In rotating evaporation temperature for 60℃conditions and pH= 7.4. This method of the preparation of fructusγ-schizandrin element of liposomes doxorubicin particle distribution of 50～200 nm. Average particle size value is (135.2±19.6) nm (n=3). Measured by flocculation separation fructusγ-schizandrin doxorubicin liposomes encapsulation efficiency can reach(96.45±1.86)%, Zeta Potential for(-38.6±2.5) mV. Results show that fructusγ-schizandrin doxorubicin can significantly improve the liposome drug concentration of plasma adriamycin. Fructus schisandrae meal can enhance the party of adriamycin, efficacy, together they can prolong drug use in the retention period of blood, to get plenty of time to get to target sites, increases healing done.5. For acute toxicity tests, fructus schisandrae b grain doxorubicin liposomes LD50=52.8 mg/kg, Doxorubicin liposomes LD50= 41.5 mg/kg, Doxorubicin LD50= 1.82 mg/kg. We can see that, Fructusγ-schizandrin to significantly reduce the toxicity of adriamycin. Using animal S-180 solid tumors model, adopt tail intravenous drug laws fructusγ-schizandrin doxorubicin liposomes anti-tumor effect. Results show that, Meat and edible bliposome fructusγ-schizandrin element has certain antitumor function but not significant, doxorubicin liposomes compared with adriamycin, Cancer-fighting improve also was not significant. But fructusγ-schizandrin doxorubicin anti-cancer effects of liposomes significantly higher than doxorubicin.6.γ-schizandrin extract had effective scavenging scavenging effect of.OH more than the same concentration of Vc, the IC50 is 0.2 mg.mL-1, scavenging effect of O2.- less than Vc, the IC50 is 0.24 mg.mL-1.γ-schizandrin extract against Staphylococcus aureus, Candida albicans, Salmonella, E. coli, Bacillus subtilis has a certain extent, of which Candida albicans and Staphylococcus aureus in the strongest inhibition, minimum inhibitory concentration of 0.25 mg.mL-1; on Rhizopus, Aspergillus niger, Nanyang yeast had no significant antibacterial activity. |
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