| Purpose:To investigate the effect and histopathologic characteristics of radiofrequency ablation (RFA) combined with transcatheter arterial chemoembolization (TACE) and RFA combined with transcatheter arterial embolization (TAE) for rabbit VX2hepatic tumors.Materials and Methods:Twenty four Japanese White rabbits with VX2hepatic tumors were randomly divided into four treatment groups:TACE+RFA, RFA followed15min later by TACE (n=6); TAE+RFA, RFA followed15min later by transcatheter arterial embolization (TAE) with poly vinyl alcohol particles (PVA)(n=6); RFA, RFA alone (n=6); and the TACE group, TACE alone (n=6).Results:The maximum diameters of the coagulation or infarcted zones in the gross specimens were compared. A rabbit in TAE-RFA group died of intraperitoneal bleeding and a rabbit in RFA group died of pneumothorax in the procedure and a rabbit in TACE+RFA group died of hepatic necrosis2days after the procedure. The procedures were successfully performed in the remaining animals (21/24;87.5%). Significantly larger areas of coagulation and infarction were observed in the TACE+RFA group compared with the TAE+RFA (P<0.05), RFA (P<0.05) and TACE groups (P<0.05). The TAE-RFA group showed larger treatment areas compared to the RFA group (P<0.05). Histopathological analysis revealed greater vascular thrombosis and necrosis and fewer islands of viable tumor cells in the TACE+RFA group compared with the other groups.Conclusions:The largest treatment dimensions were produced in the TACE+RFA group by the synergistic effect of local chemotherapy, hyperthermia and reduction of the blood flow feeding the tumors. TACE+RFA are more effective for achieving tumor destruction than TAE+RFA, RFA or TACE performed alone. Purpose:To establish over-expression HSP70model in a rabbit model of VX2hepatic tumors by TAE combined with RFA treatments.Materials and Methods:Japanese White rabbits (75animals) with VX2liver tumors were randomly divided into five groups:TAE+RFA, RFA followed15min later by transcatheter arterial embolization (TAE) with polyvinyl alcohol particles (PVA)(n=15); RFA, RFA alone (n=15); TAE group, TAE alone (n=15); Sham group, received sham procedure without radiofrequency ablation; Control group, received no procedure until sample collection time (n=15). Five rabbits in each group were sacrificed on day1,3,7after procedures, respectively. HSP70expression was detected by immunohistochemistry staining and Western blot analysis; HSP70mRNA expression was by RT-PCR.Results:HSP70expression in the liver and residual tumor in TAE+RFA group were significantly greater than others groups on dayl,3and7, respectively. HSP70expression in the liver and residual tumor in TAE+RFA group on day3had statistically significant compared with on day1,7respectively. TAE induced greater HSP70expression than control group and sham group in residual tumor on dayl. In TAE, RFA and TAE+RFA groups, HSP70mRNA expressed in the both tissues peaked on day1, dropped on day3and dropped to base line on day7after treatment. TAE+RFA and RFA groups showed a stronger HSP70mRNA expression compared with others groups in both tissues on day1.Conclusions:The expression of HSP70was noted in residual tumors and liver tissue in the marginal area of necrosis area due to different treatments, increased on day1, peaked on day3and dropped on day7after treatment in TAE, RFA and TAE+RFA groups. Hypoxia caused by TAE can enhance HSP70expression in residual tumor. RFA combined with TAE showed the synergistic effect on HSP70expression. Purpose:To explore the relationship between the expression of HSP70surrounding residual tumor and in peripheral blood and CD8+T cells surrounding the residual tumors and in peripheral blood in a rabbit model of VX2hepatic tumor after TAE+RF A treatment.Materials and Methods:Japanese White rabbits (54animals) with VX2liver tumors were randomly divided into three groups:TAE+RFA, RFA followed15min later by TAE (n=18); RFA, RFA alone (n=18); Control group, received no procedure until sample collection time (n=18). Six rabbits in each group were sacrificed on day1,3,7after procedures, respectively. HSP70expression and infiltration of CD8+T cells surrounding the residual tumors were detected by immunohistochemistry staining; HSP70expression and the positive percentage of CD8+T cells in lymphocyte in the peripheral blood was detected by ELISA Kit and measured by flow cytometry, respectively.Results:HSP70expression in the residual tumors in TAE+RFA group was significantly greater than others groups on day1,3and7, respectively; RFA group was significantly greater than Control group on day1,3and7, respectively. HSP70expression in the residual tumors in TAE+RFA, RFA groups on day3had statistically significant compared with on day1,7respectively. The number of infiltration of CD8+T cells surrounding the residual tumors in TAE+RFA group was significantly greater than others groups on day1,3and7, respectively; RFA group was significantly greater than Control group on day1,3and7. The number of infiltration of CD8+T cells surrounding the residual tumors TAE+RFA and RFA groups on day3had statistically significant compared with on day1,7, respectively. The serum HSP70levels were on peak in RFA and TAE+RFA groups on day1and the peak levels of CD8+T cells in RFA and TAE+RFA groups in peripheral blood at same time.Conclusions:There were positive correlations between HSP70expression levels and infiltration of CD8+T levels surrounding the residual tumors and in RFA, TAE+RFA groups. The ability of serum HSP70initiating of antitumor immunity may reduce with serum HSP70decreasing. TAE+RFA treatment can activate the strongest antitumor immunity by hyperpyrexia and hypoxia induction of the most significant HSP70proteins expression from residual tumors cells in rabbit VX2hepatic tumors. |
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