Study On The Bioactive Components In The Sesame, Radix Angelicae Pubescentis, Green Coffee Bean,and Birch Bark

The boiactive components in the sesame, radix angelicae pubescentis, green coffee bean, and birch bark were studied in this research. The analytical method for the quantitative determination and purification process of these constituents has been developed. Many separation problems of bioactive composition have been solved, which will forge a solid basis for natural products. Moreover, the solid-liquid equilibrium of betulinic acid in pure solvents and mixed solvents has been discussed.The main contents could be summarized as follows:The preface includes the research’s purpose, method, process and working, the document’s source, the frame of the theory and the thesis.In the second chapter, simultaneous determination of sesamin and sesamolin in sesame by high performance liquid chromatography has been developed. The established method was successfully applied to simultaneous determination of the two analytes in ten different brands of sesame oils which were purchased from different companies and batches. Furthermore, sesamin was obtained through saponification and chromatography with silica gel. A pilot test showed that this process was applied to a large-scale production.In the third chapter, we established an HPLC method to simultaneously determine three bioactive components of radix angelicae pubescentis. This improved method is convenient and suitable for control the quality of radix angelicae pubescentis.In the fourth chapter, we established the HPLC analytical methods of the chlorogenic acids (CGAs) in the coffee beans, evaluated the optimal extraction parameters of the CGAs from the coffee bean. In addition, the adsorption and desorption properties of CGAs on macroporous resins including AB-8, HPD-826, LS-303, LX-60, LX-17, and LX-68have been compared. LX-60resin offers the best adsorption and desorption capacity for CGAs than other resins based on the research results. The optimum parameters for adsorption were obtained. After treating with LX-60resin, the superior quality green coffee bean extract, the contents of CGAs were more than50%and caffeine was less than2%, was obtained. The results showed that LX-60resin revealed a good ability to separate CGAs. Therefore, we conclude that results in this study may provide scientific references for the large-scale CGAs production.In the fifth chapter, the solubilities of betulinic acid in thirteen commonly used organic solvents and THF+water mixed solvents were determined using HPLC method at different temperatures. The solubilities data was correlated with a semi-empirical equation and UNIQUAC equation. The calculated solubilities showed excellent agreement with the experimental data. The thermodynamic properties of the solution process and the crystal habit of betulinic acid were also discussed. Furthermore, we developed a novel method to separate betulin and betulinic acid from birch bark. This method is simple, cost effective, and has commercial feasibility.In the last chapter, all of the experimental work was summarized. We find the deficiencies in this thesis and propose the idea of the latter research.