Co/o < Sub > 2 < / Sub > Mixed Gas Dry Preservation Method In The Experimental Study Of The Application Of Allogeneic Nerve Transplantation

PurposeTo build up an experimental model of nerve allotransplantation with rats and to determine the feasibility of this model in accessing rejection and nerve regeneration Methods36Lewis rats served as recipients and were divided randomly into two groups, the experiment group (E) and control group (C). Their right sciatic nerves were cut off by10mm. In group E, the sciatic nerves of18Lewis rats were fixed with15mm allografts which were harvested from nine F344rats. In group C, the sciatic nerves of18Lewis rats were fixed with15mm isografts which were harvested from nine Lewis rats. The results were assessed at postoperative one, four and twelve weeks, including the indexes of ethology, electrophysiology, histopathology and immunology. ResultsAt postoperative one, and four weeks, in group E, the immunology indexes, including serum IL-2concentration, serum IL-6concentration and the ratio of CD4+T cells vs CD8+T cells were statistically higher than those in group C. At postoperative four and twelve weeks, in group E, the indexes of ethology, electrophysiology, histopathology were better than those in group C, most of which with statistical significance. ConclusionThe experimental model of transplanting sciatic nerve from F344rats to Lewis rats was good at serving as an experiment model nerve allotransplantion. PurposeTo build up experimental model of nerve allotransplantation with rats, and by applicating the methods of preserving peripheral nerves in desiccating conditions with a mixture of carbon monoxide and oxygen gases in this model, to investigate the ability of this methods in reducing the immunogenicity of the nerve allograft and promoting nerve regeneration. Methods72Lewis rats served as recipients and were divided randomly into four groups. Their right sciatic nerves were cut off by10mm. In group A, the sciatic nerves of18Lewis rats were fixed with15mm isografts which were harvested from nine Lewis rats. In group B, the sciatic nerves of18Lewis rats were fixed with15mm allografts which were harvested from nine F344rats. In group C, the sciatic nerves of18Lewis rats were fixed with15mm isografts which were harvested from nine Lewis rats and preserved in a mixture of carbon monoxide and oxygen gases for three weeks. In group D, the sciatic nerves of18Lewis rats were fixed with15mm allografts which were harvested from nine F344rats and preserved in a mixture of carbon monoxide and oxygen gases for three weeks. The results were assessed at postoperative one, four and twelve weeks, including the indexes of ethology, electrophysiology, histopathology and immunology. ResultsBetween group A and B, the results of immunology index at postoperative one, and four weeks, and the indexes of ethology, electrophysiology, histopathology at postoperative four and twelve weeks have statistical significance. Between group B and D, the results of immunology index at postoperative one, and four weeks, and the indexes of ethology, electrophysiology, histopathology at postoperative four and twelve weeks have statistical significance.ConclusionPreserving peripheral nerves in desiccating conditions with a mixture of carbon monoxide and oxygen gases could reduce the immunogenicity of the nerve allograft and promote nerve regeneration in experimental model. Nerve allograft is an alternative to autograft for repairs with nerve gapping at the injury site. Nerve allograft causes immunological rejection, which limits the regeneration of nerve axon and the recovery of nerve function. In order to reduce the immunological rejection and improve the recovery of nerve function, lots of studies were carried out. In this report, the literature of nerve allograft transplantation experimental studies, strategies to prevent nerve allograft rejection, and reported clinical experiences are reviewed.