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Study On Resource And Quality Of Medicinal Plants In Aesculus Linn

Posted on:2014-01-09Degree:DoctorType:Dissertation
Country:ChinaCandidate:Z H ShiFull Text:PDF
GTID:1224330398454123Subject:Basic Theory of TCM
Abstract/Summary:PDF Full Text Request
Aesculus (Aesculus Linn.) for Sapindales hippocastanaceae(Hippocastanaceae) deciduous trees, mainly distributed in Asia,Europe, America, with about30species,10species of china. Theplants in the origin of folk do Qi medicine, Chinese chestnut(Aesculus chinensis Bunge var.chinensis), Zhejiang Buckeye(Aesculus chinensis Bunge var.chekiangensis) and Aesculuswilsonii (Aesculus wilsonii Rehd) is the dried ripe seed is usedin traditional Chinese medicine " Chinese Medicine Dictionarycontains the Buckeye ", soothing the liver, regulating qi, andfunctions stomach pain, pesticides. Modern medical research showsthat, Sara Ko has a definite effect on anti-tumor, anti cerebraledema, the treatment of hypertensive cerebral hemorrhage, coronaryheart disease, Meniere’s disease, bronchial asthma in the elderly,facial neuritis. At present, has developed freeze-dried powderinjection, liniment, compound gel, tablet formulations, productionscale reached2,000,000,000yuan. At present, Aesculus chinensismedicinal resources in wild, with people’s understanding ofAesculus chinensis medicinal value deepened, on the increasingtheir demand for resources, resulting in wild resources have been seriously damaged, the yield of a declining trend, and wild yieldinstability, a small points, can not meet the demand of the market,the purchase price Sara Ko rose again and again; in addition thecommercial medicinal materials appear mixed varieties, quality isnot a problem, the reasonable application to Aesculi drug safety,bring huge risk.Based on the system of access to domestic and foreign Buckeyeespecially Buckeye literature resources, chemical constituents,pharmacological action, clinical application and industrialdevelopment on the subbase source plants, we found that involveslittle related research resources and quality of this genus, thereis no systematic study on the Buckeye resources distribution, anddevelopment the rational use of resources and the quality ofmedicine effective control method. Therefore, this paper focuseson Buckeye Buckeye origin resource situation, subbase source plantssuitable for the system of regional planning, DNA bar codeidentification of species, chemical quality comparison andfingerprint features of other suitability and planting, providescientific basis for effective control of the sustainabledevelopment of the medicinal resources and varieties products ofdrug.By means of literature investigation and on-the-spotinvestigation method for the first time in China, Aesculus species,distribution of suitable habitat, resource investigation andanalysis, preliminary to determine the scope of distribution withinthe Buckeye distribution in China about latitude21°32′N39°55′N,103°11′E by degrees to120degrees12’E, altitude50m1776m, warm climate, and deep, fertile, moist but well drained soil. Because wild horse chestnut resources due to uncontrolleddeforestation and the dry weather, damage is very serious, very few,be imperative of planting and system specification.Integrated field sampling, sample inspection and literatureresearch results, analysis platform of herbal origin suitableapplication (TCMGIS-II) to analyze the ecological region of theoriginal species Aesculi three radicals, determine the Buckeyeecological similarity of95%100%of the area is mainlydistributed in15in Shandong Province, Shanxi, Hebei544counties(city)(City), total suitable area is407692.4km2, which issuitable to Shandong area and county (city) accounted for thelargest proportion (suitable area is108209.1km2, accounting forthe county (city) ratio of77.4%, the same below), followed byShaanxi (85064.8km2,47.2%), Hebei (42529.4km2,44%); Aesculuswilsonii ecological similarity95%100%areas are mainlydistributed in12provinces of Guizhou, Sichuan, Hubei (city)450counties (city), total suitable area is251765km2, which issuitable for the largest area of Guizhou (55980km2,28.4%);Zhejiang Buckeye ecological similarity between95%100%of thearea is mainly distributed in9provinces Zhejiang, Hunan, Anhui(city),247counties (city), total suitable area is67569.5km2,which is suitable to Zhejiang the largest area (22395.4km2,23%),the sustainable use of resources, for the cultivation of Aesculuschinensis To provide a scientific basis and scientific division.Through the investigation of10species in the genus Aesculus42samples ITS, ITS2, psbA-trnH, rbcL, matK sequence of PCRamplification and sequencing efficiency, intraspecific andinterspecific variation, the identification efficiency, was analyzed by barcoding gap test and NJ tree to cluster the sequenceafter screening, psbA-trnH sequence bar code sequence for theidentification of Aesculus medicinal plants, and sequencing of thePCR amplification efficiency was100%, a minimum variance isgreater than the intraspecific variation, and the identificationof candidate sequences for maximum efficiency, the barcoding gaptest results show that the sequence among species, intraspecificvariation weight ratio is smaller, and the NJ tree cluster analysisresults also show that the psbA-trnH sequence effect analysis canprovide sufficient, provides a new method for rapid and accurateidentification of medicinal plants of genus Aesculus chinensis.Method for determination of ginsenoside A seven saponins fromleaves, seven leaves of B application of HPLC method to establishthe different varieties in Fructus Aesculi two index component,content by UV-visible spectrophotometric method for thedetermination of seven leaves saponins, and the effects of moisturein the sample, the total ash content, comparison of Aesculus maintypes of resources Aesculi medicinal chemical quality, kinds andcontents of different varieties in Fructus Aesculi seven leaves,seven leaves saponins saponin A B each are not identical, with atotal of seven leaf saponin content and moisture, total ash andother conventional physicochemical experiment results, Aesculuschinensis medicinal chemical differences in quality of differentvarieties of larger, providing theory and the experimental basisfor the different varieties of Aesculi medicinal comprehensivequality evaluation system.At the same time, according to the different origin of Aesculusmedicinal plants for chemical composition analysis, fingerprint analysis method to establish the quality evaluation of medicinalmaterials by Luo Zi living liquid chromatography. The AgilentZORBAX SB-C18column, acetonitrile-0.2%phosphoric acid solutionas mobile phase in gradient elution, flow rate was1mL·min-1, thedetection wavelength was220nm, the fingerprint analysis of Hubei,Shanxi, Zhejiang, Yunnan, the36batch of Aesculus chinensismedicinal plant collection, to establish the fingerprint of thechemical composition of36different areas of Aesculus chinensisgenus of herbs, and provide reliable basis for quality evaluation.For further utilization of medicinal resources, this paper alsoto Buckeye Seed oil yield as index, orthogonal test method,extraction of Suo Luo from semen Aesculi in supercritical CO2optimal extraction oil and the use of GC-MS component analysis oncomponents of semen Aesculi oil. Take the extraction pressure28Mpaand temperature38℃, the separation conditions of reactorpressure12Mpa, temperature40℃, separation of reactor pressure5Mpa and temperature40℃and the optimum extraction time110min,Buckeye Seed oil yield is1.264%. On the Buckeye Seed oil wasanalyzed by GC-MS,26compounds were identified, mainly fatty acidcomposition. Compared with petroleum ether extraction,supercritical CO2extraction has high yield, short extraction time,fatty oil clarification etc. For the two kinds of extraction methods,fatty acid composition of higher oil content in the basic agreement,the fatty acid composition of low content difference.The plant resources and quality system study on the genus ofAesculus chinensis, and provides technical support and scientificbasis for the resources of the medicinal plants of Aesculusscientific development, rational utilization and sustainable development.
Keywords/Search Tags:Aesculus, suitability, DNA code, fingerprint, qualityresearch
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