The Feasibility Study For A New Hemostatic Agent Prepared By The Electrospun Nanoifbers With Thrombin Loaded

Each of the commercially available hemostatic products has its own uniquecharacteristics, and, on the other hand, has its own limitations. Therefore, it isnecessary to develop the more effective hemostatic materials. The electrospinningtechnique has become an effective method to produce a drug delivery system inrecent years. It is an appealing way to produce a rapid hemostatic agent throughloading the thrombin on the electrospun materials. In this study, we evaluated theefficacy and the feasibility regarding the new hemostatic agent which prepared bythe electrospun thrombin-loaded nanofiber-based materials.ObjectivesTo explore the feasibility and the properties of a new homemade hemostaticagent prepared by electrospun nanofiber membrane with thrombin loaded.Methods1. The optimal polymers was determined, and the experimental hemostaticagents were prepared via electrospinning combined with thrombin loaded.2. The stability examinations of the experimental hemostatic agents wereperformed in different conditions including the room temperature, the high or lowtemperature, the acid or alkaline environment, and the highlight exposureenvironment.3. Comparative study about the efficacy in hemostasis was performed indifferent hemorrhagic animal model among the experimental hemostatic agents,the absorbable gelatin sponge, and the oxidized cellulose.ResultsThe experimental hemostatic agents obtained a better enzyme activitymaintaining in low temperature environment, whereas, the acid or alkalineenvironment and the highlight exposure led to inactivation of the thrombin thatwas loaded on the electrospun membrane. The experimental hemostatic agentswere more effective than absorbable gelatin sponge for controlling bleeding afterhepatic trauma in the animal model. Meanwhile, its reliability of hemostasis in the arterial hemorrhage model was better than the oxidized cellulose. In the errhysismodel after abdominal aorta repair, the experimental hemostatic agents were lesseffective than the oxidized cellulose.ConclusionsThe electrospun thrombin-loaded hemostatic agent is a promising product inthe clinical application in future.