| PurposeThe detail of this study:the subject is selected on the basis of literature, rat stroke models were used to measure the efficacy of actupuncture especially the JIN’3needle on the stroke. Rat survival, the extent of neurological function’s damage, serum lactate dehydrogenase and others were compared between the conventional group, model group to provide experimental evidence for acupuncture treatment of stroke research.MethodsThe rats were divided into conventional group, acupuncture group and model group. Acupuncture group and model groups were used line leash method to suture the MCA to make stroke modle, during the period of surgery, maintain body temperature at37±5℃.10%chloral hydrate in the dosage of3ml/kg in the way of intraperitoneal to anesthesia the rats, neck midline incised, separated of the left common carotid artery, external carotid artery, internal carotid artery and internal carotid artery external carotid branches pterygopalatine artery. Ligation of the common carotid artery proximal end, the starting end of the external carotid artery and pterygopalatine artery end, the bolt of the common carotid artery was cannulated line, the line tied with a single strand of nylon line, pre-tagged scale in the nylon line head-end mill obtuse, and soaked with10%L-lysine. Carotid fork as the starting mark the insertion depth was1.8±0.5cm, to date there was a slight sense of resistance, truss artery stump skin was sutured. Sham operation group operation and the operation is the same, but do not plug wire. Postoperative animals caged with.Screening modeling successful in24h after acupuncture treatment. This experiment, all other points in accordance with the Huaxing Bang "animal acupuncture points map" acupoints. Acupuncture group to the temporal three-pin main points with three-pin of hand, foot three needles, acupuncture treatment by the same person, the needle for30min, during which every10min line pin1. Acupuncture every other day, to pin3. The conventional group acupoints:upper extremity of shoulder skull, Qu pool, foreign relations, gu, lower extremity take Huantiao, Yang Ling Quan, Zusanli, Xie River, Kunlun, the way the use of acupuncture affected side, with lift and thrust twisting reinforcing each1min. Model group by acupuncture, clinical signs observed, the survival rate of rats and neurological symptoms, brain water content and the degree of edema, serum lactate dehydrogenase determination, were used in this observation.Resultsclinical signs observed:body weight190-210g, fur smooth, rosy lips nose and mouth, a good mental state, active fighting, aggressive, good diet, dry stools. The suture method after surgery, the rats withered hair, spiritual malaise, weight decreased, reduced activity, offensive weakened, reduced food intake, pale green nose and mouth, loose stools. Post-operative the hemiplegia symptoms:the left front paw is weak, the left front paw is not received within a circular motion to the left, crawling, body tilted to the left.(2) rat survival and neurological symptoms:three treatment acupuncture group survival rate after surgery was significantly higher than the model group, sham operation group without neurological deficit, model group and acupuncture group ischemia2h nerve function defect was no significant difference ((P>0.05). model group at24h after reperfusion neurological deficit compared with ischemia2h aggravated the acupuncture group reperfusion24h neurological deficit that has been restored, at each time observation points compared with model group with significant difference (P<0.05)(3) brain tissue water content and the degree of edema:model group brain water content was significantly higher with the other groups, indicating that the model can lead to an increase in brain water content; Acupuncture amount of group water content lower than the model group.(4) Determination of serum lactate dehydrogenase (LDH):A week later, the LDH content of the model group than the sham-operated group was significantly higher (p<0.5), the LDH content of the acupuncture group lower than the same period the model group (p<0.01).(5) serum ACL-IgG levels were measured results show that the model group level of serum aCL-IgG content was significantly higher than in the sham-operated group (p<0.05), further surgical modeling success. Acupuncture group aCL-IgG level was significantly lower than the model group (p<0.01).(6) Determination of serum fibrinogen results show that the model group animal serum fibrinogen was significantly higher than in the sham-operated group (p<0.05), further surgery successful modeling. Fibrinogen content of the acupuncture group compared with model group tended to decrease, but the difference was not significant (p>0.05).ConclusionThe method of line leash to make ischemic stroke rat models, stroke symptoms were appeared after surgery, the rat model of different degrees of paralysis symptoms:the left front paw is weak, the left forelimb can not adduct circling to the left, crawling, even the body tilted to the left. This method was Simple operation, and modle easily success. Acupuncture has a protective effect on focal cerebral ischemia neurological, can reduce rat mortality, improve neurological symptoms, reduced brain water content and the degree of edema, serum lactate dehydrogenase is also reduced. Acupuncture can treat and have a good effect to improve stroke aspect. |
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