Effect Of Hign Protein Food On Expression Of CD68, MCP-1 And TNF Alpha In Renal Tissue Of Rats

In recent years, the incidence of metabolism and nutrition-related diseases (diabetes, hyperuricemia, etc.) is increasing year after year because of the combination of elevated living standards, change in the eating habits. High protein can cause kidney damage, leading to progressive deterioration of renal function in chronic kidney disease (CKD) patients. Under normal physiological conditions, high protein diet will result in kidney damage, and how its mechanism,more study should be done to reveal it.Objective:To investigate the effect of high protein food on the expression of CD68, MCP-1 and TNF alpha in renal tissue of rats.Methods:According to body weight,24 Wistar rats were randomly divided into two groups, fed with basic diet and high-protein diet for 12 weeks. Feeding and drinking water freely.24 h-U-ALB,24 h-U-TP,24 h-U-Cr, FBG,Scr,BUN,TC,TG,Ccr were determined at week 0,4,8 and 12. The rats were sacrificed 12 weeks later.The renal pathological changes were examined and the expression of CD68, MCP-1 and TNF alpha in renal tissue was detected.Result:1. Body weight of rats. The body weight of rats has been increasing over time. But the body weights of two groups had no statistically significant differences during experiment(P> 0.05).2.Blood glucose and blood lipids of rats. The FBG,TC,TG of rats has been increasing over time. But the FBG,TC,TG of two groups had no statistically significant differences during experiment(P> 0.05).3. Proteinuria of rats. The 24 h-U-ALB,24 h-U-TP of rats has been increasing over time. But the 24 h-U-ALB,24 h-U-TP of two groups had no statistically significant differences during experiment(P＞ 0.05).4.The renal function of rat. The BUN,Ccr of rats has been increasing over time. But the BUN,Ccr of two groups had no statistically significant differences during experiment(P> 0.05). The Scr of rats has been decreasing over time. But the BUN,Ccr of two groups had no statistically significant differences during experimen(P> 0.05).5.The diameter of glomerular. The diameter of glomerular of two groups had no statistically significant differences after 12 weeks (P> 0.05).6.The expression of CD68, MCP-1 and TNF alpha. After 12 weeks, the expression of CD68,MCP-1 and TNF alpha of experimental group were markedly increased as compared with that of control group (P< 0.05),especially on tubules and interstitial.Conclusion:1. High-protein feeding Can not change the weight and the level of FBG,TC,TG,24 h-U-ALB,24 h-U-TP,BUN,Ccr,Scr.2. High protein feeding can increase expression of MCP-1 to promote the filtration of monocytes/macrophages and produce more TNF alpha,and induce the inflammation of renal.