Experimental Study And Clinical Evaluation Of Invigorating The Spleen, Regulating The Liver And Resolving Dampness On Simple Obesity With Impaired Glucose Regulation (IGR)

Nowadays, obesity has become one of the fastest-spreading and the most seriouspublic health problems worldwide. The prevention and treatment of obesity has becomethe most significant public health problem in the world in the21st century.Professor XuYunsheng has discovered that stagnation of liver-QI with deficiency of the spleen as thefundamental morbidity of simple obesity, should use the invigorating the spleen andregulating theliver treatment，and founded JianPiTiaoGanYin. This study through twoaspects of clinical and experimental observe the effect and mechanism of JianPi-TiaoGanYin.The content of the research is as follows.Objective:Animal experiment:The obese with IGR model of50wistar rats were induced byusing high fat feed. The successful modeling rats were included in the experiment.Treatment using JianPiTiaoGanYin and metformin. To observe obesity-related indicators,glucose and lipid metabolism indicators of liver tissue morphology, adipokines,gastrointestinal hormone, serum Nesfatin-1and its expression in gastric mucosa, etc., andfurther explore the possible mechanism of action and contact.Clinical trial: Obese subjects were randomly grouped,To observe the changes of bodyweight,lipid metabolism, serum Nesfatin-1, etc in patients with simple obesity, Evaluatedeficiency clinical efficacy and safety of treatment on simple obesity with JianPi-TiaoGan. Method:Animal experiment: a randomized control method to molding good rats were dividedinto3group, model group, JPTGY group, MET group, given different treatment methodsrespectively, To observe body weight, body length, Lee’s index, FPG,HOMA-IR,bloodlipid, serum LEP,TNF-α, IL-6, Nesfatin-1, and its expression in gastric mucosa.Clinical trial: Patient participated in the observation were diagnosed as simpleobesity，Then randomly grouped to2groups: Experimental group and control group.Experimental group was treated by JianPiTiaoGanYin,1Agents a day, divided into twoparts. The control group was treated by metformin,0.25g taken30minutes before meals,three times a day. Experiment take period12weeks.To observe To observe body weight,Waist circumference, WHR, TC, TG, HDL-C, LDL-C, FPG, FINS, HOMA-IR, serumNesfatin-1. Evaluate deficiency clinical efficacy and safety of treatment on simpleobesity with JianPiTiaoGan.Results:Animal experiment: JianPiTiaoGanYin can effectively slow down the rate of weightgain of rats (P<0.01), reduce abdominal fat wet weight (P <0.01), improvement of insulinresistance (P <0.01), and lower serum triglyceride levels (P<0.01) and improved lipidmetabolism;Also can effectively reduce simple obese rats’hepatic steatosis and possibly byreducing serum Leptin (P<0.01), TNF-α levels (P<0.01) to improve chronic inflammationand insulin resistance.JianPiTiaoGanYin also can effectively increase serum Nesfatin-1levels (P<0.01) and increased its expression in gastric mucosa.Clinical trial: JianPiTiaoGanYin can reduce the simple obesity patients‘BMIassociated (P<0.01), reduce the central obesity (P<0.05); also improve insulin resistance(P<0.05), significantly reduced TG (P<0.01), having similar effects with metformin (P>0.05); Further, JianPiTiaoGanYin could significantly elevated levels of serum Nesfatin-1(P <0.01) and improved quality of life (P <0.01).Conclusion:1. JianPiTiaoGanYin can effectively reduce body weight, reduce the central obesity,regulate glucose and lipid metabolism, improve insulin resistance, reduce hepatic steatosis,improve the state of chronic inflammation in obese rats and raised serum Nesfatin-1leveland its expression in the gastric mucosa.2. JianPiTiaoGanYin can effectively reduce the simple obesity with IGR patientsweight and improve central obesity and its symptoms.improve quality of life. Also canreduce serum triglyceride levels, increased serum Nesfatin-1levels and improve insulin resistance conditions. The mechanism on regulation of lipid metabolism of simple obesitypatients may through upward Nesfatin-1levels.This study observed the efficacy and possible mechanism of JianPiTiaoGanYin as thetreatment of simple obesity through animal experiment and clinical trial, verify thecorrectness and feasibility of therapeutic interventions.Also provides a TCM treatment’snew way of thinking and methods of simple obesity.