Experimental Study On Hemostasis Of Thermosensitive Chitosan Hemostatic Film

Medical biomaterials are a class special materials of medical functions, they can beapplied to the special function in clinical, widely applied in various tissues and organs,improved disease diagnosis, and is beneficial for treatment of patients with the disease, andno adverse effects on human tissue. The biological hemostatic materials are one kind of thebiomedical materials. Currently, there are a large number of hemostatic biomaterials, butsome no hemostatic effect, some allergic reaction, some expensive, etc. The chitosan hasgood hemostatic effect, and good tissue compatibility, low price. In our experiments, thechitosan has good hemostatic effect, but it is weak adhesion with wound. we modifiedchitosan using chitosan main chain active2-NH2and6-OH, reformed the formulation ofchitosan and changed the structural of chitosan, and get the chitosan gel for temperaturesensitive, then the chitosan gel was frozen,dried, made into suitable size, thickness film.The thermosensitive chitosan film had strong adhesion with wound. But if thethermosensitive chitosan hemostatic film is good hemostatic, it would needed to experimentto be confirmed. we would investigated the hemostasis and biocompatibility of the chitosanhemostatic film through animal experimentation and provided the basis for nextapplications.PART ONEEXPERIMENTAL STUDY ON HEMOSTASIS OF THERMOSENSITIVECHITOSAN HEMOSTATIC FILMObjective To study the hemostasis of thermosensitive chitosanhemostatic film. Methods Thirty-six adult SD rats were divided into three groupsrandomly,every group was12rats. The rats were made the models of liver injury,theincision was covered different hemostatic materials. The incision of the liver was coveredthermosensitive chitosan hemostatic film (group A), cellulose hemostatic film (group B), and blank control (group C) respectively. The incision of the liver was covered with thehemostatic materials of2.0cm×1.0cm×0.5cm. The bleeding time and bleeding amountwere recorded. Results Gross observation showed good hemostatic effect and fasterhemostatic time in groups A and B; group C had no hemostatic effect. The bleeding time ofgroup A, B and C was (1.50±0.32),(1.52±0.38), and (4.83±1.20) min, the bleeding amountwas (0.68±0.08),(0.71±0.10),(2.25±0.36), respectively. The bleeding time and bleedingamount of groups A and B were significantly lower than those of group C (P <0.01). but nosignificant difference was found among groups A and B (P>0.05). Conclusion Thehermosensitive chitosan hemostatic film has good hemostasis effect. PART TWOEXPERIMENTAL STUDY ON HEMOSTASIS OF DIFFERENT DOSAGE FORMSCHITOSAN HEMOSTATIC MATERIALObjective To investigate the hemostasis of different dosage formschitosan hemostatic material. Methods Seventy-two adult Sprague Dawley rats wererandomly divided into6groups, every group was12rats. They were made the models ofliver injury and the models of femoral artery injury. The incision of the liver was coveredwith the hemostatic materials of200mg, thermosensitive chitosan hemostatic film (groupA1), chitosan hemostatic film (group B1) and chitosan hemostatic powder (group C1),respectively. The bleeding time and bleeding amount were recorded. The incision of thefemoral artery was covered with the hemostatic materials of500mg, thermosensitivechitosan hemostatic film (group A2), chitosan hemostatic film (group B2) and chitosanhemostatic powder (group C2), respectively. The bleeding time and bleeding amount wererecorded. Results Gross observation showed good hemostatic effect and faster hemostatictime in groups A1and A2, thermosensitive chitosan hemostatic film became gelatinousblood clot in the incision of the liver. Groups B1, C1, B2and C2had weak hemostatic effect and slower hemostatic time. The bleeding time and bleeding amount of groups A1wassignificantly lower than those of groups B1and C1(P <0.05). But no significant differencewas found among groupsB1and C1(P>0.05). The bleeding time and bleeding amount ofgroups A2was significantly lower than those of groups B2and C2(P <0.05). But nosignificant difference was found among groupsB2and C2(P>0.05). Conclusion Thethermosensitive chitosan hemostatic film has good hemostasis effect. PART THREEEXPERIMENTAL STUDY ON HEMOSTASIS DEGRATION AND TISSUECOMPATIBILITY OF THERMOSENSITIVE CHITOSAN HEMOSTATIC FILMObjective To investigate the hemostasis, degradation and tissue compatibilityof thermosensitive chitosan hemostatic film. Methods Thirty-six adult SD rats wererandomly divided into3groups, every group was12rats. The SD rats were made themodels of liver injury and the models of quadriceps femoris muscle injury. The incision ofthe liver was covered with the same size hemostatic materials, thermosensitive chitosanhemostatic film (group A1), cellulose hemostatic cotton (group B1), and gelatin sponge(group C1), respectively.The bleeding time and bleeding amount were recorded. Theincision of the quadriceps femoris muscle was embedded with the hemostatic materials of0.3cm×0.3cm×0.3cm, thermosensitive chitosan hemostatic film (group A2), cellulosehemostatic cotton (group B2), and gelatin sponge (group C2), respectively. After one week,two weeks, three weeks, four weeks, six weeks, the incision tissues of the liver and thequadriceps femoris muscle were cut for observation of material absorption and tissuemorphology. Results Gross observation showed good hemostatic effect and fasterhemostatic time in groups A1and B1. Group C1had weak hemostatic effect and slowerhemostatic time. Groups A1and A2tissues adhesioned lightly.After6weeks, thethermosensitive chitosan hemostatic film was absorbed completely. Groups B1and B2 tissues adhesioned lightly.After3weeks, the cellulose hemostatic cotton was absorbedcompletely. Groups C1and C2tissues adhesioned severely.After2weeks,the gelatin spongewas absorbed completely. There were more new cells and less inflammation of groups A1and A2through microscope, the TEM (transmission electron microscopy) showed that theliver cell nucleus and organelles of group A1were more integrity.Conclusion The thermosensitive chitosan hemostatic film has good hemostasis effect andtissue compatibility.