Study On Application Of Chitosan Dressing And Genotoxicity

Chitosan is the most important derivative of chitin, widely found in the shrimp, crabs, insects, animal and plant, is very rich in nature, the preparation method is simple, inexpensive. High density chitosan can achieve weight loss, lowering blood pressure, the purpose of lowering blood pressure, is widely used in the health care products and pharmaceutical additives; Water-soluble chitosan is soluble in water, better absorption, moisturizing, antibacterial function, applicable to fields such as cosmetics, food, water treatment, fruit and vegetable preservation, pharmaceutical preparations, dyeing sewage; Chitosan have cosmetic chitosan,flocculant chitosan, tobacco chitosan, agriculture, feed, bait chitosan. Chitosan has a non-toxic, anti-oxidation, bio-degradable, antigenicity, biocompatibility, etc. Are considered to be the most promising material one in translational medicine applied to the skin tissue.First, we take advantage of the laboratory has been established in animal models, using the technology to detect various chitosan dressing hemostatic properties of the rat abdominal surgery. And implanted in rats chitosan dressing observed degradation effect in a certain time, chitosan dressing in the extent of degradation in the body, pathological observation.The subject combined the bleeding the chitosan dressing wound repair and liver hemostasis experimental animal model using rat peritoneal surgery to detect a variety of chitosan dressings to stop bleeding performance. Meanwhile, we detected on rat sperm observed chitosan dressings adversely affect whether germ cells. The results are as follows:1. Through animal experiments to observe the effect of N-fructose modified chitosan quaternary ammonium derivativeson rat skin wound healing. Results display the time of wound healing there is significant difference between the experimental group and control group(P<0.05); Wound healing area and volume of healing the extent of the experimental group and the control group between the two groups was significant(P<0.05);In conclusion:N-fructose modified chitosan quaternary ammonium derivatives does not harm the skin, promote skin healing, suitable as a skin repair materials,is an ideal raw material for medical dressing.2. Vivo observation homemade chitosan sponge(Group A) commercially available chitosan Preparation the Sponge (Group B), as well as the control group gelatin sponge (Group D) hemostatic effect, evaluation homemade chitosan hemostatic dressing hemostatic effect. Statistical methods to compare the hemostatic effects of chitosan and gelatin sponge the two chitosan sponge hemostatic effect than gelatin sponge, administered the chitosan sponges rats its bleeding quantity, significantly less than the application of gelatin sponge ratsthe amount of bleeding. Laboratory synthesis of chitosan hemostatic effect better than the market to buy chitosan sponge. Two homemade chitosan sponge, the vampire rate is less than the gelatin sponge. Tip chitosan sponge is the ideal material for future hemostatic dressings instead of gelatin sponges.3. Homemade chitosan sponge (Group A) commercially chitosan sponge(Group B), as well as the control group gelatin sponge (Group D) in vivo observed in vivo degradation. And evaluation3the effect of the implant material in vivo degradation. The results show homemade chitosan sponge and market purchases chitosan sponge implantation of rat liver after a period of time, the implant material is not completely degraded, and implantation of the degradation rate of the absorbable gelatin sponge than the other two groups fast. Description In this stage, clinical application of absorbable gelatin sponge as an implant material is optional, chitosan sponges as implant materials still in development stage. There is no doubt that the physical and chemical properties of the chitosan is the ideal choice for implant materials.4. By animal experiments, observe homemade chitosan sponge(Group A), market purchase chitosan preparation the Sponge (Group B), and gelatin spongc(Group D) rat germ cells, evaluation planted into materials play a mutagenic effect on sperm. The results show that Normal rat sperm deformity rate is the lowest compared with the control group homemade chitosan sponges, the market purchase chitosan sponge absorbable gelatin sponge malformation rate was significantly lower than the control group (P<0.05). Chitosan sponge and gelatin sponge does not induce rat sperm deformity, no mutagenic effect on germ cells in rats.