Exogenous ATP Improve The Embryo Adhesion And Implantation By Acting To The P2Receptors Which Lead To The Intracellular [Ca2+] Up-regulation

Embryo implantation is a mysterious and delicate process which include the embryo apposition, adhesion and invasion to the endometrium, with many adhesion factors, growth factors and inflammatory factors involved, and these processes have great connections with the intracellular free calciunm([Ca2+]) up-regulation. We studied the effects of exogenous ATP and the P2receptor antagonist Suramin on the JAr embryo adhesion model and and ICR embryo implantation model and found10-5M concentration of ATP can significantly increase the adhesion rate of the JAr model while Suramin can significantly decrease the adherion rate; in the ICR model we injected ATP of Suramin in the cornua uteri and found a similar result. We detected the intracellular [Ca2+] of endometrial epithelium cell (Ishikawa cell) and trophoblast cell(JAr cell) up-regulated by the ATP stimulation with a concentration ranging from10"8M to10’5M. It’s well known that intracellular [Ca2+] up-regulation triggers series of expression and regulation of adhesion factors,growth factors,inflammatory factors and their receptors. So we conclude that ATP can improve the embryo adhesion and implantation by activating the P2receptors which lead to the intracellular [Ca2+] up-regulation and the downstream signaling.