| Osteoporosis is mainly divided into primary and secondary, primary besides idiopathic, divided into type I and type II, also known as postmenopausal osteoporosis, type I to type high conversion, the main reason for the lack of female hormones; Type II, also known as senile osteoporosis, for the low transformation type, due to the aging of age. Osteoporosis caused by many factors, it is the basic pathological mechanism of bone resorption and bone formation in bone metabolic processes coupling appear defects, lead to calcium phosphorus metabolism imbalance in the body, to gradually reduce the clinical symptoms caused by bone mineral density. Clinical main show is: pain, fracture, length shorten, humpback, decreased respiratory function, etc. Especially fracture, osteoporosis fracture occurs more in reverse body, content, fenestration interior everyday activities, even if there is no obvious larger external force, can fracture. Fracture location for thoracic and lumbar vertebral body, distal radius, and upper femur.Bone biomechanical properties is to evaluate the quality of the bone a direct, objective and reliable indicators. Skeletal adaptive response to the function of movement to stimulate, actually including the bone structure, bone mass and mechanical properties of three aspects, namely the movement to stimulate increased bone strain, increase bone mass and bone structure improvement.Traditional Chinese medicine "kidney advocate bone marrow", "taste is the foundation of the day after tomorrow" "blood stasis caused by bone impotent" theory, think the disease is associated with kidney and spleen, and kidney essence, the bone marrow, the diurnal long into stasis kidney empty, kidney damage, long illness treatment with kidney and promoting blood circulation to remove blood stasis, kidney I give priority to.Therefore, to formulate the movement combined with traditional Chinese medicine compound animal model of castrated rats, to explore the movement and influence of Chinese traditional medicine compound for the movement based on the mechanism of action of the efficacy of traditional Chinese medicine for its improvement. Experimenta 1 movement combined with traditional Chinese medicine compound bone mineral density and biomechanics of castrated rats and the effects of ultrastructureBuilding 12 weeks, take out on the right side of the femur, bone mineral density experiment results show that the model group rats bone mineral density value compared with the control group was extremely significant difference(P ﹤ 0.05), movement, Chinese traditional medicine compound group has significant difference compared with model group(P ﹤ 0.05), exercise combined with traditional Chinese medicine compound groups have significant difference compared with model group(P ﹤ 0.01). In biomechanical experiments, according to the results of model group rats femur maximum load compared with the control group was extremely significant difference(P ﹤ 0.01), exercise group, supplies, sports combined with traditional Chinese medicine compound group has an extremely significant difference compared with model group(P﹤0.01). Under the light microscope and electron microscope results show that the movement in combination with prevention and treatment of osteoporosis, the best effect of traditional Chinese medicine compound group. Experiment 2 sports combined with traditional Chinese medicine compound ALP of castrated rats, the Ca/P and E2, NOS, OPG related factorBuilding after 12 weeks, ELSIA experiment results show that, compared with model group, exercise group on the testing results of ALP, E2 no statistical significance(P<0.05), the Ca/P is no statistical significance(P<0.05); Compared with model group, control group, the traditional Chinese medicine compound, sports combined with traditional Chinese medicine compound group in ALP detection has significant difference(P<0.05), E2 detection were extremely significant differences(P<0.01), the Ca/P there was no significant difference(P<0.05); Control NOS, OPG value compared with model group, with significant difference(P<0.05), the exercise group compared with model group had significant difference(P<0.05), Chinese medicine compound, sports combined with traditional Chinese medicine compound group compared with model group had extremely significant difference(P<0.01).Experiment three sports combined with traditional Chinese medicine compound of OPG and RANKL protein expression and the influence of m RNABuilding after 12 weeks, the experimental results show that the model group were the value of OPG and RANKL and control group with significant difference(P < 0.05), sport, Chinese medicine compound group and model group was extremely significant difference(P< 0.05), sports combined with traditional Chinese medicine compound group and model group had extremely significant difference(P< 0.01); RT-PCR, according to the results of the experiment control RANKLm RNA expression compared with model group rats was extremely significant difference(P ﹤ 0.01); Movement, Chinese traditional medicine compound group compared with model group had significant difference(P﹤0.05); Exercise combined with traditional Chinese medicine compound group compared with model group had extremely significant difference(P﹤0.01).Four movement experiment combined with traditional Chinese medicine compound of castrated rats skeletal muscle mitochondria, skeletal muscle protein content and SOD activity changeThe right side of the building 12 weeks after quadriceps experimental results show that the mitochondrial activity control group compared with model group had significant difference(P<0.05), movement, Chinese traditional medicine compound group compared with model group had significant difference had statistical significance(P<0.05), exercise combined with traditional Chinese medicine compound group compared with model group had extremely significant difference was statistically significant(P<0.01); In terms of skeletal muscle protein content, control group had the extremely significant difference compared with model group,(P﹤0.01) of traditional Chinese medicine compound group compared with model group had significant difference(P﹤0.05), movement and exercise combined with traditional Chinese medicine compound group compared with model group had extremely significant difference(P﹤0.01); the rat serum SOD content changes, according to the results of the control group compared with model group, the difference was statistically significant(P<0.01), sport, Chinese medicine compound group difference was statistically significant(P<0.05), sports combined with traditional Chinese medicine compound group compared with model group, the difference was statistically significant(P<0.01). |
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