The Effect Of The Nephritis Xiaobaikeli Towards The Prodocyte-Relating Proteins Of The Rats Of Diabetic Nephropathy

Objective:With the observation of the effects of nephritis Xiaobaikeli towards the urinary in 24 hours,kidney,cell factors,prodocyte-relating proteins (nephrin, podocin),Pathway of the rats of diabetic nephropathy (DN) at the early stage in experimental research,is mechanism in delaying the development of DN is investigated.Method:After Unilateral nephrectomizing the models of rats of diabetic nephropthy at the early stage with High Fat/High Sucrose Diet and Streptozotocin, they are aeparated into 5groups:the blank control group, the model contrast group, the group with high dosage of nephritis Xiaobaikeli, the group with low dosage of nephritis Xiaobaikeli and the group with Benazepril Hydrochloride (Lotensin). After 8 weeks, test the weight of the rats, their weight of kidney blood sugar, blood serum creatinine (Scr), blood urea nitrogen (BUN) and UAE:Color the Pathological Sections PAS, observe the Pathological changes of the kidney of the rates:look into the expression of cell factors IGF and VEGF with the Immunohistoche mistry; test the expression of nephrin-mRNA and podocin-mRNA through Semi-quantitative RT-PCR; test the expression level of nephrin and podocin with Western Blot.Result:Nephritis Xiaobaikeli fails to ameliorate the weight of the rats of diabetic nephropathy at the early stage, their weight of kidney blood sugar, blood serum creatinine (Scr) and blood urea nitrogen (BUN),with no distinctive difference compared with the model group (P>0.05); Nephritis Xiaobaikeli can reduce the weight of the kidney and decrease its index:it can reduce the urine UAE evidently, the group with nephritis Xiaobaikeli is much clearer than the model group(P<0.05), it can lighten the Mesangisl Proliferation, Basement Membrane incrassation and the fuse of foot process; it can decrease the expression of IGF,VEGF of the rats kidney, with distinctive difference compared with the contrast group(P<0.05); it can increase the expression of nephrin-mRNA and podocin-mRNA of the kidney, both with distinctive difference compared with the contrast group(P<0.05); it can increase the expression level of nephrin,podocin with Western Blot, both with distinctive difference compared with the contrast group(P<0.05).Conclusion:Nephritis Xiaobaikeli can relieve the kidney index of rats of diabetic nephropathy at the early stage, with the increasing of the expression of prodocyte-relating proteins nephrin and podocin, the reducing of the expression of rats’kidney cell factors IGF and VEGF and the reducing of the discharging of the Proteinuria, the purpose of protecting kidney is abstained, at the same time, the diabetic nephropathy of the rats is delayed.