Associations Between Three Lipid-related Gene Polpmorphisms And Coronary Artery Disease And Ischemic Stroke

Part â… Association of polymorphisms in the MAFB gene and the risk ofcoronary artery disease and ischemic strokeBackground: MAFB has been associated with serum lipid levels in the Eurpean population. However, little is known about such association in the Chinese population or in atherosclerosis-related patients.Objective: The purpose of the present study was to assess the association of the single nucleotide polymorphisms(SNPs) in the MAFB and serum lipid levels and the risk of coronary artery disease(CAD) and ischemic stroke(IS) in the Chinese population.Methods: A total of 1,065 unrelated patients(CAD, 525 and IS, 540) and 539 healthy controls were recruited in this study. Genotypes of the MAFB rs2902940 and rs6102059 SNPs were determined by the Snapshot technology platform.Results: The AA genotype of rs2902940 SNP was associated with an increased risk of CAD(adjusted OR = 1.63, 95% CI = 1.07-2.48, P = 0.023) and IS(adjusted OR = 1.69, 95% CI = 1.09-2.61, P = 0.017). The GA/AA genotype was also associated with an increased risk of CAD(adjusted OR = 1.56, 95% CI = 1.04-2.32, P = 0.030 for GA/AA vs. GG) and IS(adjusted OR = 1.72, 95% CI = 1.14-2.60, P = 0.010 for GA/AA vs. GG). Significant interactions were observed only in those with higher BMI, hypertension and diabetes(P<0.05). The subjects with GA/AA genotypes in controls had lower serum Apo AI levels than the subjects with GG genotype(P = 0.024).Conclusions: The A allele carriers of SNP rs2902940 in the MAFB conferred a decreased serum Apo AI levels in controls and an increased risk of CAD and IS. The GA/AA genotypes interacted with higher BMI, hypertension and diabetes to contribute the risk of CAD and IS.Part â…¡Association of polymorphisms in the FADS1/FADS2 gene cluster andthe risk of coronary artery disease and ischemic strokeBackground: Little is known about the association of the FADS1/FADS2 single nucleotide polymorphisms(SNPs) with serum lipid levels and the risk of coronary artery disease(CAD) and ischemic stroke(IS) in Chinese southern population.Objective: The present study aimed to detect the association between the FADS1/FADS2 SNPs and serum lipids levels and the risk of CAD and IS in Chinese southern population.Methods: A total of 1,669 unrelated subjects(CAD, 534; IS, 553; and healthy controls, 582) were recruited in the study. Genotypes of the FADS1 rs174546 SNP and the FADS2 rs174601 SNP were typed by SNa Pshot Multiplex Kit.Results: The T allele and TT genotype frequencies of the two SNPs were predominant in Chinese south population(P < 0.05). The T alleles were associated with increased risk of CAD and IS(for rs174546 SNP, OR = 1.30, 95% CI = 1.06-1.59 for CAD, and OR = 1.29, 95% CI = 1.06-1.57 for IS; for rs174601 SNP, OR= 1.38, 95% CI= 1.13-1.69 for CAD, and OR = 1.42, 95% CI = 1.16-1.73 for IS). Correspondingly, carrying C alleles were associated with reduced risk of CAD and IS(for rs174546 SNP, OR = 0.57, 95% CI = 0.44-0.75 for CAD, and OR = 0.62, 95% CI = 0.48-0.80 for IS; for rs174601 SNP, OR = 0.54, 95% CI = 0.41-0.70 for CAD, and OR = 0.61, 95% CI = 0.48-0.79 for IS). Haplotype analyses showed the haplotype of T-T(rs174546-rs174601) was associated with an increased risk for IS(OR = 1.32, 95% CI = 1.10-1.59), and the haplotype of C-C(rs174546-rs174601) was associated with a reduced risk for IS and CAD(OR = 0.72, 95% CI = 0.60-0.87 for CAD, and OR = 0.76, 95% CI = 0.63-0.91 for IS). Carrying T allele carriers of the two SNPs and rs174601 TT genotype were associated with decreased serum HDL-C and Apo AI in the patients groups(P < 0.05).Conclusions: The present study suggests that the FADS1 rs174546 SNP and the FADS2 rs174601 SNP are associated with the risk of CAD and IS, and are likely to influence serum lipid levels.Part â…¢Association of ANGPTL4 polymorphisms with the risk of coronaryartery disease and ischemic strokeBackground: Angiopoietin-like protein 4 gene(ANGPTL4) has been associated with serum lipid traits in the European population. However, the association of the ANGPTL4 single nucleotide polymorphisms(SNPs) with serum lipid profiles and atherosclerosis-related diseases has not been previously detected in the Chinese population.Objective: The purpose of the present study was to assess the association of the ANGPTL4 SNPs and serum lipid levels and the risk of coronary artery disease(CAD) and ischemic stroke(IS) in the Han Chinese population.Methods: A total of 1,654 unrelated subjects(CAD, 568; IS, 537; and healthy controls, 549) were recruited in this study. Genotypes of the rs4076317, rs7255436, rs1044250 and rs2967605 SNPs were determined by the Snapshot technology platform.Results: The CT/TT genotype of rs2967605 SNP was associated with a decreased risk of CAD(adjusted OR = 0.68, 95% CI = 0.47-0.99, P = 0.043 for CT/TT vs. CC) and IS(adjusted OR = 0.55, 95% CI = 0.38-0.80, P = 0.020 for CT/TT vs. CC). Significant interactions were observed only in gender, body mass index, alcohol consumption and diabetes(P < 0.05). There were no significant effects of the four SNPs on angiographic severity of CAD in different genetic models(P > 0.05). The subjects with the rs4076317 CG/CC genotype in controls had higher total cholesterol(TC) and low-density lipoprotein cholesterol(LDL-C), and lower high-density lipoprotein cholesterol(HDL-C) levels than the subjects with the GG genotype(P < 0.0125).Conclusions: The CG/CC genotypes of the ANGPTL4 rs4076317 SNP are correlated with high TC and LDL-C, and low HDL-C levels in controls, and the rs2967605 CT/TT genotypes were associated with a decreased risk of CAD and IS. The observed associations suggest that the ANGPTL4 SNPs have a potential role on serum lipid levels and atherosclerosis-related diseases in the Chinese population.