Association Of The DNAH11 Rs12670798 Single Nucleotide Polymorphism And The Risks Of Coronary Artery Disease And Ischemic Stroke

Objective: Cardiovascular and cerebrovascular diseases have become the first cause of death in the world. Different parts of the incidence of coronary artery disease (CAD) and ischemic stroke (IS), but they have common pathophysiological basis, two disease predisposing conditions and risk factors.Our previous studies have showed that the rs 12670798 single nucleotide polymorphism (SNP) in the DNAH11ene (DNAH11) is associated with serum lipid levels in the general populations. The present study was undertaken to detect the associations between the rs 12670798 SNP and the risk of coronary CAD and IS in the Guangxi Han population.Methods: This study recruited 1,108 unrelated patients (CAD, 568 and IS,540) and 541 healthy controls from the First Affiliated Hospital, Guangxi Medical University. The diagnosis of CAD was based on typical clinical symptoms, electrocardiographic changes, increased serum markers including creatinine kinase-MB and troponin T, and coronary angiographic findings(coronary stenosis≥ 50% in at least either one of the three main coronary arteries or their major branches such as diameter ≥ 2 mm). The classification of IS was made according to the TOAST (Trial of Org 10172 in Acute Stroke Treatment)criteria. Genotypes of the rs 12670798 SNP were determined by the Snapshot technology platform.Results: Serum total cholesterol (TC) levels in healthy controls were different among the three genotypes of rs 12670798 SNP (P < 0.05), the rs1670798C allele carriers had higher TC than the C allele non-carriers;respectively. The rs12670798C allele carriers were associated with an increased risk of CAD (rs1670798CT genotype: OR = 1.345, 95%CI = 0.975-1.855; CC genotype: OR = 1.590, 95%CI = 1.109-2.278, P < 0.05). The rs12670798C allele carriers were also associated with an increased risk of IS (CT genotype:OR = 1.597, 95%CI = 1.153-2.213; CC genotype: OR = 1.722, 95%CI =1.192-2.488, P < 0.05). After adjustment for age, gender, body mass index(BMI), smoking, drinking and hypertension, the rs12670798 SNP was still associated with an increased risk of CAD and IS in different genetic models (P <0.05). Stratified analysis showed that the SNP may interact with the gender, age,BMI, smoking, drinking and hypertension to affect (increase or decrease) the risks of CAD and IS.Conclusions: DNAH11rs12670798 SNP is associated with elevated serum TC levels, and increased risk of CAD and IS in the Guangxi Han population.The rs12670798C allele carriers have higher serum TC levels and higher risk of CAD than the rs12670798TT homozygotes. DNAH11 rs12670798 SNP is also associated with the susceptibility of IS, the rs12670798CC homozygote is associated with an increased risk of IS. There may be an interaction between the rs 12670798 SNP and gender,age,BMI, smoking, drinking and hypertension to influence the risk of CAD and IS.