| Background- Several carrier beads were used for control release of antibiotic drugs in osteomyelitis treatment, but these non-biodegradable materials has less potential of releasing desired amount of drug in-vivo, so this new approach to repair bone defect with control sustained release of levofloxacin from Nanosphere for osteomyelitis treatment, we compared HPLC & UV-VIS spectrophotometry technique to analyze the in vitro release of drug.Objective- To compare the HPLC & UV-VIS spectrophotometry technique for control release of levofloxacin from nanosphere in treatment of osteomyelitis.Material & Methods- Nanosphere containing antibiotic drug(levofloxacin) were analyzed by using two techniques HPLC & UV-VIS spectrophotometry. Standard curve was obtained. Releasing concentration of drug was measured.Result- The developed methods illustrated excellent linearity(r2 > 0.999) in the concentration range of 0.5- 50 μg/ml and 0.5- 250 μg/ml for UV spectrophotometric and HPLC methods, respectively. HPLC showed mean of total drug release 92.72 ± 31.10 μg/ml on mean concentration of 30.91 ± 10.37 μg/ml, while UV-VIS showed mean of total drug release 563.79 μg/ml on mean concentration of 187.932 μg/ml respectively.Conclusion- Comparing the released amount of drug by both techniques showed that, HPLC is more potential technique for in-vitro drug release detection through biodegradable nanosphere as compared to UV-VIS spectrophotometry. HPLC is more specific technique for in-vitro drug assay than UV-VIS spectrometry. |
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