Study On Quality Control Standards Of Two Medicinal Plants Beesia Calthifolia And Dendrobium Officinale

Studies of Beesia calthifolia and Dendrobiμm officinale have been increasing nowadays while the quality-control standards of them remain insufficient, which has become obstacle for the ways of further development and utilization. The current study focused on the quality standardization of the two medicinal materials by development and usage of modern instrumental analysis methods. Quantitative analysis methods for total triterpenoids and three triterpenoid saponins have been established by using the ultraviolet-visible spectrophotometry and high performance liquid chromatography (HPLC)-evaporative light-scattering method (HPLC-ELSD) techniques, respectively. Parameters for solvent extraction of polysaccharides from D. officinale have been optimized by orthogonal array tests. Furthermore, an ultraviolet spectrophotometry method has been established and applied to quantiative analysis of the stilbenoids content of D. officinale. Technological process for extraction and purification of stilbenoids and polysaccharides have been further studied. Here in, the main research contents were listed below:1. Quantitative analysis method for total triterpenoids of B. calthifolia was established on the basis of the glacial acetic acid-vanillin-perchloric acid chromogenic method. After methodology validation, this method has showed good specificity, stability, repeatability and accuracy, which can meet the criteria for quantitative analysis and can be applied for quantitative determination of the total triterpenoids content.2. An analytical method for quantitatively and simultaneously determination of the contents of three representative triterpene glycosides from B. calthifolia has been established for the first time by HPLC-ELSD technology. Methodology validation has showed good specificity, stability, repeatability, and accuracy, which can meet the criteria for quantitative analysis. This method could be used for content determination and quality standard of B. calthifolia.3. Single factor experiments and orthogonal experiments were carried out to evaluate the influence of extraction temperature, pH value, amount of extraction solvent, extraction time, extraction times on the extraction rate of D. Officinale polysaccharide.The best process conditions are:12 times the amount of boiling water extraction for 2 times,2 hours each time. Validation experiments resulted in a stable and feasible polysaccharides extraction rate around 5.98%.4. An analytical method for quantitatively determination of the contents of stilbenoids from D. officinale has been established for the first time by UV spectrophotometry. Single factor and orthogonal experiments were carried out to determine the influence of extraction solvent, extraction method, ethanol concentration, extraction temperature, extraction time, liquid-material ratio, and extraction times on the quantitative analysis_of stilbenoids from D. officinale powder. The best process conditions for sample preparation are:70 times the amount of 50% ethanol as extraction solvent, extracting for 3 times and 3 h each time. The methodology validation experiments showed high accuracy and feasibility for this method, and suitable for quantitative analysis. This method was also applied for content determination of stilbenoids from different batches of D. officinale.5. Taking decoction pieces of D. officinale (dried stem segments) as research object, single factor and orthogonal experiments showed the influence of the amount of solvent times, ethanol concentration, extraction temperature, and extraction time on the extraction rate of stilbenoids. The best conditions for extraction process are:12 times the amount of 80% ethanol as extraction solvent, extracting twice at 92℃ for 3 h the first time and 2 h for the second. Validation experiments resulted in a stable and feasible polysaccharides extraction rate around 3.02%.6. Successively extraction sequence of polysaccharides and stilbeoids were studied and showed no significant difference of overall extraction rate. Ethanol first and water second is the ordinary order.