Application Of The Chinese Herbal Drugs Composite Materials In Trauma Repairs

This dissertation reviewed research progress of salvia miltiorrhiza in promoting burn wound recovery and the culture and clinical application of epidermal cells and summarized chemical composition and anti-oxyradica injure, protecting endothelial cell, improving microcirculation, promoting tissue repair, inhibiting excessive regenerate and immunoregulation function of salvia miltiorrhiza. This paper also summarized resource of epidermal cells, separation of epidermal cells, cultural method of epidermal cells, clinical application ofepidermal cells and view its development. In addition, applications of nanotechnology in biomedical engineering and biological security of nanomaterials were reviewed.Objective: To construct salvia miltiorrhiza /nano-silver/ chitosan film composite. Investigate the efficacy and bio-safety of salvia miltiorrhiza /nano-silver/ chitosan film composite and adherence, growth, proliferation of keratinocytes on this composite. Method: Using nanometer and self-assembly technology to construct salvia miltiorrhiza/nano-silver/ chitosan film composite. Sterility test, pyretogen test, primarily skin stimulus test, intradermally stimulation test, acute general toxicity test were performed to investigate the bio-safety of this composite. The efficacy of salvia miltiorrhiza/nano-silver/chitosan filmcomposite was tested in in vivo experiments on deep partial-thickness wound created on Sprague Dawley rats (SD rats). Therapeutic effect was evaluated through macroscopic observation, calculating healing ration and pathological section. In addition, the concentration of silver in SD rats’ blood and other tissues were determined by flame atomic absorption spectrophotometry (FAAS); Keratinocytes were cultured on salvia miltiorrhiza/nano-silver/ chitosan film composite. 6h, 12h, 24h after inoculation, the cell attached rations were calculated respectively. The cultured cells identified and characterized by immunohistochemistry and transmissive electron microscope (TEM). Results: Salviamiltiorrhiza/nano-silver/chitosan film composite was sterility, no pyretogen, no stimulus to skin, no general toxicity. 10 and 13 post scald day, the wound healing rates were 90.65±4.03 % and 98.98±5.04 % respectively in salvia miltiorrhiza /nano-silver/ chitosan film composite group. This was higher than control groups (P＜0.05) and SD rats curing by this composite were free from infection. The silver level in blood was lower than SD-Ag group (P＜0.01). 13 post scald day, the blood silver content in salvia miltiorrhiza/nano-silver/chitosan film composite returned to normal level, while in SD-Ag group, the silver content was 5 times than normal level(P＜0.05). The concentration of tissue silver was higher than normal level in salvia miltiorrhiza/nano-silver/ chitosan film composite and SD-Ag group. But the silver level in tissues in SD-Ag group was much higher than composite group (P＜0.01). Salvia miltiorrhiza/nano-silver/chitosan film composite could significantly (P＜0.01) increase the attached ration of keratinocytes. 24h after inoculation, the attached ration of keratinocytes were 65.55±3.01% and 28.61±2.23 %on this composite and cell culture plastic, respectively. After inoculation 12h, 24h, the attached rations on this composite were the highest. However, the attached ration after inoculation 6h on this composite was lower than that on chitosan film. Meanwhile, there were not any fibroblasts growing on this composite. Conclusion: Salvia miltiorrhiza/nano-silver/chitosan film composite was sterility, no pyretogen, no stimulus to skin, no general toxicity. It can accelerate wound healing and decrease the risk of silver poisoning. In addition, it could significantly promote keratinocytes attached ration and effectively inhibited the growth of fibroblasts. The rapidly proliferating keratinocytes, which were cultured on this composite, maintained their bioactivity. Its good efficacy and bio-safety shows a prosperous future in clinical setting. This indicates that the composite was nontoxic and was a good candidate for wound dressing in skin tissue engineering.