The Study On Hypoxia Exercise Inducing MiR-27/PPARγ,miR-122/PPARβ To Regulate Lipid Metabolism In Obese Rat’s Liver

Objective: Study the impact of different exercise modes on the level of mi R- 27 / PPAR gamma, mi R- 122 / PPAR beta and lipid metabolism target gene and protein expression level in the obesity rat liver through experiment, and study the sequential effect of hypoxia exercising on the level of mi R- 27 / PPAR gamma, mi R- 122 / PPAR beta and lipid metabolism target gene and protein expression level in the obesity rat liver. Explore the regulation mechanism of obesity in the rat liver lipid metabolism through the induction of hypoxic exercise on mi R- 27 / PPAR gamma, mi R- 122 / PPAR beta.Methods: 80 obese rats were randomly divided into eight groups(n=10, no obvious difference between groups in terms of weight): normoxic group(N group),exercise group(E group),hypoxia group(L group),hypoxia and exercise group(L+E group)/ 4-week hypoxia and exercise group(E4 group), 0-week hypoxia and exercise group(C group), 1-week hypoxia and exercise group(E1 group), 2-week hypoxia and exercise group(E2 group), 3-week hypoxia and exercise group(E3 group). All groups had endurance exercising on the treadmill with 25 m/min in normal oxygen concentration or 20 m/min in hypoxia(oxygen concentration is 13.6%, equivalent to altitude of 3500 meters), 4 weeks’ exercise with 1 h/d and 5 d/w.Results: Effects of different exercising modes on physiological and biochemical levels of obese rats:Exercise,hypoxia, hypoxia exercise decreased weight, adipose tissue around the kidney weight, epididymal adipose tissue weight, fat/body weight ratio, BMI, Lee’S index, TC concentrations, TG concentrations, LDL-C concentrations in obese rats. In contrast, three kinds of exercising modes increased HDL-C concentrations. Effects of different exercising modes on levels of mi R- 27 / PPAR gamma and lipid metabolism factor in obese rats’ liver: hypoxia exercise decreased the expression of mi R-27 in obese rats. Exercise,hypoxia, hypoxia exercise increased the expression of PPARγ、CYP7A1、CD36、SREBP1,but no significant influence on ABCA1、ATGL、LPL、L-FABP. Effects of different exercising modes on levels of mi R- 122 / PPARβ and lipid metabolism factor in obese rats’ liver: Exercise,hypoxia, hypoxia exercise decreased the expression of mi R-122. In contrast, three kinds of exercising modes increased the expression of PPARβ、CPT1、FAS、ACC.(2)The sequential effect of hypoxia exercising on physiological and biochemical level of obese rats’ : hypoxia exercise stedily decreased weight, adipose tissue around the kidney weight, epididymal adipose tissue weight, fat/body weight ratio, BMI, Lee’S index, TC concentrations, TG concentrations, LDL-C concentrations in obese rats. In contrast, it stedil increased HDL-C concentrations. The sequential effect of Hypoxia exercising on levels of mi R-27 / PPARγ and lipid metabolism factor in obese rats’ liver: hypoxia exercise stedily decreased the expression of mi R-27. In contrast, it stedil increased the expression of PPARγ、CYP7A、CD36、ATGL、LPL. L-FABP expression level was highest in the E1 group. ABCA1 expression level was highest in the E2 group. SREBP1 expression level was no significant change. The sequential effect of Hypoxia exercising on levels of mi R-122/ PPARβ and lipid metabolism factor in obese rats’ liver: hypoxia exercise stedily decreased the expression of mi R-122. In contrast, it stedil increased the expression of PPARβ、CTP1. ACC expression level was highest in the E3 group. FAS expression level was no significant change.Conclusions:(1) Exercise, hypoxia, hypoxia exercise can increase the speed of cholesterol efflux and fatty acid oxidation, improve their blood liquid level, decrease the body fat of obese rats, and the effect of hypoxia exercising is most significant.(2)Hypoxic exercise may be decrease the mi R – 27 and mi R – 122 level in the liver of obese rats, and negatively regulate the expression of PPARγ and PPARβ, and affects the dynamic balance of fat metabolism in the obese rats’ liver, then leads to the decrease of visceral fat of obese rats.(3)Hypoxia exercise regulates the expression of CD36, ATGL, LPL, PPARβ, CPT1 and ACC after the transcription in obese rats’ liver.