Analysis Of Genetic Ddiversity And Distribution Of Catalpa Bungei And Catalpa Fargesii In China

Catalpa bungei and Catalpa fargesii had already been cultivated more than2000years inChina, and has valuable utilization which use its wood, leaf, flower and fruit. To protect and usethe germplasm resources of Catalpa bungei and Catalpa fargesii in China, this thesisinvestigated the germplasm resources all over the countrywide, built GIS database, studieddistribution status, and used ISSR and SRAP molecular marker methods to analyze group geneticdiversity. Additionally, the thesis compared leaf-utilization components among different cultivars,types and clones of Catalpa bungei and Catalpa fargesii. The main results were summarized asfollows:(1) Originally,19province have Catalpa bungei and Catalpa fargesii old trees or stands.Geographical distribution space were within north latitude5°8＇～40°26＇and east longitude99°26＇～122°2＇. Meteorological factors, site type, slope type, soil, water, traffic status andhuman disturbance all influenced the distribution area and amount of Catalpa bungei andCatalpa fargesii trees.(2) Different species, provenances and individuals of Catalpa bungei and Catalpa fargesiihad significant variation in leaf, flower, fruit and stem phenotypic characteristics. Variation ofleaf length, leaf width, petiole length, anthocaulus length, fruit length and thousand kerner weightis bigger than those of seed length, seed width, diameter of pollen.(3) ISSR and SRAP molecular marker methods can be used to analyze the group geneticdiversity of Catalpa bungei and Catalpa fargesii plant. ISSR used14primers to do PCR,percentage of polymorphic was77.78%, UPGMA cluster analysis showed6group types amongthe total samples. SRAP used6primers to do PCR, percentage of polymorphic was90.79%,UPGMA cluster analysis showed7group types among the total samples.(4) Leaf of Catalpa bungei and Catalpa fargesii can be used as energy feed, but not suit tobe protein feed. Among14materials, there had significant difference in soluble sugar, solubleprotein, water, chlorophyll, and coarse fat content. Clones of Fangongting, Taiqinggong,Qingzhensi, and cultivar Yuqiu2were selected as further breeding materials.