Research On The Relationship Between Collagen Gene Polymorphisms And Cerebral Infarction And Atherosclerotic Plaque

Objective To clarify the suspended association of the collagen gene(COL3A1, COLlA2) polymorphisms with cerebral infarction and to discuss the relationship between polymorphisms of collagen gene and onset risk of atherosclerotic plaque.Methods Case control study was performed in this study. We collected cerebral infarction patients as CI group, which was divided into stable plaque subgroup and vulnerable subgroup according to carotid ultrasound examination. Then we chose healthy individuals as the control group,whose ages and sexes were matched with the CI group. There were no relationships among the subjects. We collected the case history and recorded the examination results seriously. We draw fasting venous blood from every subject early in the morning and obtained DNA from the blood sample using Blood DNA Kit. PCR-RFLP was used to detect the polymorphisms of COL3A1-SNP-rs1800255and COLlA2-SNP-rs42524of all subjects. Association of the collagen gene (rs1800255, rs42524) polymorphisms with cerebral infarction, carotid intima-media thickness and onset risk of carotid stable plaque, vulnerable plaque was analyzed.Results1.The genotypic distribution of rs1800255had significant differences between the CI group and the control group (P<0.05), and had significant differences between the A allele (AA+AG) carriers and the GG genetype carriers for rs1800255(P<0.05), but the allelic distributions of rs1800255had no significant differences between the CI group and the control group (P>0.05). Logistic regression analysis showed that any genotypes of rs1800255could not increase the risk of the onset of CI (P>0.05).2.The genotypic and allelic distributions of rs1800255had no significant differences between different CIMT level subgroups (P>0.05).3.The genetypic and allelic distribution of rs1800255had significant differences between the stable plaque, vunerable plaque subgroups and the control group (P<0.05). Logistic regression analysis showed that AA genotype could increase the risk of the onset of vunerable plaque (P<0.05).4.The genotypic and allelic distribution of rs42524had no significant differences between the CI group and the control group (P>0.05). Logistic regression analysis showed that any genotypes of rs42524could not increase the risk of the onset of CI (P>0.05).5.The genotypic and allelic distributions of rs42524had no significant differences between different CIMT level subgroups (P>0.05).6.The genetypic and allelic distribution of rs42524had no significant differences between the vunerable plaque, stable plaque and control groups (P>0.05). Logistic regression analysis showed that any genotypes of rs42524could not increase the risk of the onset of vunerable plaque (P>0.05).Conclusion1. Association of rs1800255polymorphisms with cerebral infarction in Hunan Han Population may exit. AA/AG genotype of rs1800255may play promoting actor for the onset of cerebral infarction, but it is not an independent risk factor for the onset of cerebral infarction. No association of rs1800255polymorphisms with CIMT levels exists. The AA genetype of the rs180255may be a risk factor for the formation of carotid vulnerable plaque in Hunan Han Population.2. Association of the rs42524polymorphisms with cerebral infarction in Hunan Han Population may not exist. No association of rs42524polymorphisms with CIMT levels exists. The polymorphism of rs42524has nothing to do with onset risk of carotid stable plaque or vulnerable plaque.