Myopathy Mouse Tumor Angiogenesis Research Estrogenic Effect Targets Uterine Po Ling And The Regulating Circulation And Pain Relief

Objective:take Estrogen effect correlator and angiogenesis correlator as target point, investigate the mean mechanism of the disease in different time and in different space of the uterus endomembrane; approach the mechanism of Chinese medicinal prescription of promoting qi to activate blood; compare the distinction between different therapeutic(al) does of Chinese medicinal formulae、and between the chinese medical science and Western medicineMethods:carry out an operation to transplant the Male mice pituitary gland into the uterine cavity of the female mice in order to construct the AM mice model. breed the mice for3and6months to form the the model of3months and which of6months. select clinical effective medical prescription (Chinese medicinal prescription of promoting qi to activate blood) as the experimental medicine, divide into high dose group、medium dose group and low-dose group, meanwhile take soft extract of DANEFUKANG as the control group of traditional Chinese medicine, take Gestrinone as the control group of western medicine, take mice without operation as blank group, give different drugs for3months in each group. then observe the change of Estrogen effect correlator (p450、COX-2、Era) and angiogenesis correlator(VEGF、 ENS、CD31、IMMP-2、TIMMP) by immunohistochemisty technique in different time and in different space of the uterus endomembrane.Results:1. p450:in the uterus endomembrane, the the value of p450gradually increase after the construction of mice model of AM, the group of3months is slightly higher the the blank group (P=0.258),the group of6months is exceed the group of3months (P=0.006); in dystopia endomembrane, the the value of p450in the group of6months notable higher than the group of3months. In the deferent period of the diease, the value of p450in dystopia endomembrane is dramatics higher than in the uterus endomembranThe value of p450is dramatics decrease after treatment in each group no matter in uterus endomembrane or in dystopia endomembrane. in uterus endomembrane, the value is significant lower in Gestrinone group than in soft extract of DAFK (P=0.038), slightly lower than which in high dose group. However in dystopia endomembrane,,there is no statistics difference between each group.after treatmentm, the value of p450decrease in each group contrast to the model group in uterus endomembrane, which is more higher in6months group (P=0.007) than3months group. in dystopia endomembrane, the value of p450is notable decrease in each group after treatment. The drease is dramatics in6months group (P=0.001), and in3months group as well (P=0.012), the decrease is more higher in6months group than in3months group.there is no statistics difference between different dose group wether i n uterus endomembrane or i n dystopia endomembrane2. COX-2:in the uterus endomembrane, the value of COX-2gradually increase after the construction of mice model of AM, the group of3months is slightly higher the the blank group (P=0.212),the group of6months is exceed the group of3months (P<0.001). in dystopia endomembrane, the the value of p450is slightly higher than in the uterus endomembrane, there is not statistically significant between the groups.in dystopia endomembrane,, the value of COX-2is notable higher in the group of6months than in the group of3months (P=0.001) The value of COX-2is dramatics decrease after treatment in each group no matter in uterus endomembrane or in dystopia endomembrane. in dystopia endomembrane, the value is significant lower in Gestrinone group than in high dose group (P=0.052) after treatment, in uterus endomembrane, the value of COX-2has no notable change in3months group (P=0.992), however, there is a dramatics decrease in6months group.in dystopia endomembrane, the value of COX-2has no notable change in3months group after treatment(P=0.677), however, there is a dramatics decrease in6months group. wether in uterus endomembrane or in dystopia endomembrane, after treatment, the value of COX-2decrease dramatics in high dose group than in medium、low dose group (P=0.005)(P=0.054)。3. ERa:in the uterus endomembrane, the ERa in3months is slightly lower than the blank group (P=0.470), in the6months group, the value of ERa is significant exceed the value in the group of3months (P=0.029). in dystopia endomembrane, the value in the group of6months is slightly than which in group of3months (P=0.107)。In the deferent period of the diease, the value of p450in dystopia endomembrane is dramatics higher than in the uterus endomembraneAfter treatment, the value of Era is decrease wether in uterus endomembrane or in dystopia endomembrane. in uterus endomembrane, the value is slightly lower in Gestrinone group than which in high dose group (P=0.236, P=0.309), there is no statistics difference between the two groups, there is no statistics difference between each group in dystopia endomembrane.after treatment, in uterus endomembrane, the value of ERa has no notable change in3months group, however, there is a dramatics decrease in6months group. in dystopia endomembrane, the value of ERa has a dramatics decrease in each group after treatment, which is especially dramatics in6months group.in uterus endomembrane, after treatment, the value of ERa decrease in each group, but there is no statistics difference between different dose group. in dystopia endomembrane, there is a dramatics decrease in high dose group than in medium、low dose group after treatment。4. CD31与VEGFMVD CD31is significant decrease in group of3months contrast to the group of blank group (P=0.006), after6months of the operation, CD31is slightly increase, but still significant lower than blank group (P=0.011)In the deferent period of the diease, the value of p450in dystopia endomembrane is dramatics higher than in the uterus endomembrane raise up not statistically significant significant exceed;to surpassThe value of CD31is increase after treatment in each group in dystopia endomembrane. i n dystopia endomembrane, the value is significant higher in high dose group than in Gestrinone group (P<0.001) and in soft extract of DAFK group (P<0.001). in uterus endomembrane, there is no statistics difference between each group. The value of VEGF and CD31decrease in each group after treatment in dystopia endomembrane, there is no statistics difference between he chinese medical science and Western medicine. after treatment, in uterus endomembrane, the value of VEGF dramatics decrease in each model group (P<0.001); in3months group, the value of CD31has a dramatics increase in3months group (P<0.001), however, there is no significant change in6months group (P=0.945)。. in dystopia endomembrane, the value of VEGF is significant decrease wether in3months group (P=0.018) or in6months group (P=0.102); the value of CD31has downtrend wether in3months group(P=0.238) or in6months group (P=0.087)i n uterus endomembrane, after treatment, the value of VEGF decrease in each group, but there is no statistics difference between different dose group. the value of CD31significant increase (P<0.001) after treatment which is higher in high dose group than in medium and low dose group (P<0.001) in dystopia endomembrane, the value of VEGF has a downtrend in each dose group, which is more lower in high dose group than in low dose group. The value of CD31has a downtrend in each dose group, but there is no statistics difference between different dose group.5. MMP-2、MMP-2/TIMP:in the uterus endomembrane, the value MMP-2significant increase after the construction of mice model of AM, the group of3months is higher the the blank group (P=0.012),the group of6months is exceed the group of3months (P=0.698). in dystopia endomembrane, the value in the group of6months is slightly than which in group of3months (P=0.027)。 after the operation, the value of MMP-2in each group is notable increase wether in the uterus endomembrane or in the dystopia endomembrane contrast to the blank group, which is more higher in the uterus endomembrane than in the dystopia endomembrane. the value of MMP-2/TIMP is higher in the uterus endomembrane than in the dystopia endomembrane, especially in the group of3months.Contrast to the mice model group, the value of MMP/TIMMP decrease in each group wether in uterus endomembrane or in dystopia endomembrane. Which in Gestrinone group is slightly lower than the other two group, however, there is no statistics difference between the three groups. Contrast to the mice model group, the value of MMP-2significant decrease in each group wether in uterus endomembrane or in dystopia endomembrane, the value is significant lower in Gestrinone group than in high dose group (0.001) and in soft extract of DAFK group (0.042) in dystopia endomembrane. after treatment, in uterus endomembrane, the value of MMP-2、MMP-2/TIMMP, has no notable change in6months group, however, there is a dramatics decrease in3months group. in dystopia endomembrane, there is no statistics difference after treatment in each model group.in uterus endomembrane, after treatment, the value of MMP-2decrease in group (P=0.041), which is lower in high dose group than in medium and low dose gmnp, but there is no statistics difference between different dose group. in dystopia endomembrane, there is a dramatics decrease in each dose group (P=0.002, P=0.001, P=0.001), but there is no statistics difference between different dose group. The value of MMP-2/TIMMP decrease dramatics in high dose group than in medium、low dose group after treatment。Conclusions:1. It can be presumed that the abnormality of Estrogen effect correlator angiogenesis correlator and the interaction of the two correlators bring about the development the AM, in defferent phase of the disease, the main mechanism is different.In early phase, angiogenesis correlator is the main mechanism to manage the disease. In uterus endomembrane, the descend of the MVD lead to the ischemia and anoxia in local position, and then induce the pullulation of the fast reaction factor COX-2, triggering the positive feed back loop of COX-2-PGE2-P450arom, induce the value of p450gradually increase with the progress the disease. The increase of p450lead to the raise of the value of estrogen, because of the degenerative, there is a slightly decrease of the value of ERa in uterus endomembrane.I n advanced stage, Estrogen effect correlator is the main mechanism to manage the disease. Estrogen can up-regulation the express of VEGF by receptor regulation, induce the disorder Vascular proliferation. The sensitivity of receptor descend by the continuously stimulation of the high concentration of the estrogen, which is tolerance of the estrogen. Then the level of the receptor compensatory increase, the level of ERa increase wether in uterus endomembrane or in dystopia endomembrane.2. Chinese medicinal prescription of promoting qi to activate blood treat AM by multitarget. The target of the treatment can change along with the different stage of the disease. In early phase, angiogenesis correlator is sthe main target. The Chinese medicine can improve the situation of the anoxemia, interrupt the positive feedback link directly. In advanced stage, the angiogenesis correlator is not the main mechanism any more, Estrogen effect correlator manages the progress of the disease. At the time, the main target of the Chinese medicine is Estrogen effect correlator, the change of Estrogen effect correlator conduct the change of angiogenesis correlator.3. The dose of the Chinese medicine is positive correlate to the express of CD31、VEGF、COX-2、MMP-2、MMP-2/TIMMP in uterus endomembrane, which has no notable correlation to the Estrogen effect correlator,such as p450、ERa. It can be presumed that using high dose of Chinese medicinal prescription of promoting qi to activate blood in early stage of the disease impeoves the microcirculation effectively, interrupt the early trigger mechanism. In adevanced stage of the disease, continuely using low-media dose Chinese medicine change the abnormity of estrogen effect, guarantee the healing,effect,, at the same time, reduce the dose of the medicine, responently reduce the toxic and side-effect, decrease the cost of the patient.4. Gestrinone decrease the the level of ERa in not only ectopia endomembrane, but also in uterus endomembrane. Compared to the western medicine, Chinese medicine avoids the side effect of the low estrogen symptom effectly...