| This thesis includes two parts:two literature reviews and an experimental study.The first part of the literature review was the progression of the TCM therapy of lung cancer in recent years. It was including six parts:etiology and pathogenesis, syndrome differentiation, the TCM treatment research, the syndrome differentiation and treatment of integrated traditional Chinese and western medicine, the TCM maintenance therapy in advanced lung cancer, and the mechanism of Chinese medical treatment for lung cancer. This literature review showed that integration of traditional and western medicine treatment of lung cancer emphasizes the syndrome differentiation, specimens consideration, integration of strengthening healthy energy and eliminating evil. And this kind of treatment obtained satisfied clinical effect on improving clinical symptoms, reducing chemoradiotherapy adverse reaction, enhancing the quality of life, prolonging the survival time.The second part of the literature review summarized the research in PPAR-γ signal path at present from following several aspects:protein structure and pathway, the connection with carcinoma, the investigation progress of ant i-tumor mechanism. Some of papers showed that the natural medicine may could against the cancer cells through the PPAR-γ signal path.But part of mechanism of TCM therapy for cancer is still unknown. This would be likely to create difficulties on globalizing. In order to found the potential mechanism of the Pingfei Compound, this research observed the inhibitory action of the medicinal serum/apozem of the compound to the proliferation of the human lung adenocarcinoma cell line A549, and the apoptosis of the tumor cells, the expression of Caspase-3.Purpose This research discussed the inhibitory action of the medicinal serum/apozem of the Pingfei Compound to the proliferation of the human lung adenocarcinoma cell line A549, the apoptosis and the expression of Caspase-3, through serum pharmacology methods, flow cytometer, western blot, on the basis of previous studies.Methods①erum containing Pingfei Compound was prepared by serologic pharmacology method. Pingfei Compound was administered orally to SD rats at doses of20g/kg/d. The proliferation of A549cells after exposure to the serum was measured by CCK-8assay at Oh,24h,48h,72h and96h.②Groups were blank control, liquid extract of Pingfei prescription1mg/mL,5mg/mL and10mg/mL group. Prol iferat ion of A549cells after exposure to the apozem was measured by CCK-8assay at24h,48h, and72h.③The PPAR-y specificity blocker GW9662and the Pingfei Compound were added in the cell cultures. Observe if the Pingfei Compound could influence the growth of the A549cells or not.④For apoptosis study, it was detected by analyzing plasma membrane translocation of phosphatidylserine, using FACS.⑤Caspase-3in A549cells was measured with Western Blot. Statistic analysis for quantitative data is student t-test.Results⑥The growth of A549could be suppressed at the48h after exposure to the serum from rats (P<0.05). The inhibi t ion was enhanced over time. The results exists significant difference compared with the control group at the72h and96h (P<0.01)⑦5mg/mL and10mg/mL groups presented inhibitive effect on A549growth at24h,48h and72h time points{P<0.01).⑧GW9662reversed partly the effect of Pingfei Compound on the proliferations of A549cells.⑨10mg/ml group could induse the early and late apoptosis induction (P<0.01).⑩The expression of Caspase-3was significantly increased by Pingfei Compound.Conclusion Apozem of Pingfei Compound, a TCM formula, could suppress A549growth in vitro in dose and time dependent manner. This inhibitory effect was related to its apoptosis induction. Perhaps the increase of the expression of Caspase-3induced the apoptosis. The mechanism of Pingfei Compound may could be involved in PPAR-y signal pathway. |
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