| Objective To observe the effects of pigment epithelium-derived factor (PEDF) onthe proliferation and apoptosis of human lung adenocarcinoma A549Cells.Methods PEDF of various concentrations (0,80,160,320and640nmol/L) wasused to intervene A549cells in vitro for various time (24h,48h and72h). The effectsof PEDF on cell proliferation was then assessed by MTT assay at the time point of24h,48h and72h, respectively. The rate of apoptosis induced by PEDF was determined atthe time point of48h with flow cytometry.Results With intervention by PEDF of various concentrations (0,80,160,320and640nmol/L), the rates of inhibition of A549cell proliferation were0±0.095,0.196±0.080,0.371±0.048,0.463±0.035and0.580±0.037, respectively, at the time point of24h; the rates were0±0.025,0.246±0.080,0.412±0.053,0.519±0.047, and0.642±0.034, respectively, at the time point of48h; and the rates were0±0.025,0.311±0.094,0.464±0.085,0.579±0.041and0.691±0.057, respectively, at the time pointof72h. For a certain time (24h,48h, or72h), the rate of inhibition was increased bydegrees with augmentation of PEDF concentration (P <0.05); for a certainconcentration of PEDF (80,160,320, or640nmol/L), except the rates of inhibitionbetween24h and48h were of no statistical differences for both80and160nmol/LPEDF, the rate of inhibition was generally increased by degrees with augmentation of PEDF intervention time (P <0.05). In addition, the rates of apoptosis of A549cellsinduced by PEDF of various concentrations (0,80,160,320and640nmol/L) were0.0035(blank control),0.0545,0.1003,0.1128and0.1528, respectively, at the timepoint of48h. The rate of apoptosis was increased by degrees with augmentation ofPEDF concentration (P <0.05).Conclusion PEDF may in vitro inhibit proliferation and induce apoptosis inhuman lung adenocarcinoma A549Cells. The inhibition of proliferation might beassociated with PEDF concentration and intervention time, and the induction ofapoptosis might also be dependent on certain concentration of PEDF. |
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