| Sika deer antler velvet layer is a kind of precious traditional Chinese medicine,which has been widely used for treatment of some diseases. In this paper, sika deerantler velvet which is productive in Shuangyang, China, was choosen as the object ofstudy. We are extrcting the lipid soluble components by using supercritical carbondioxide extraction. Study on the separation and purification identification ofcompound lipids such as cerebroside and ganglioside. At the same time, this paperalso studies the extraction and optimization of glucolipid substance in bovine spinalcord. The research contents are as follows:(1) Study on the extraction of cerebroside from the lipid soluble fraction of sikadeer antler velvet layer was investigated using response surface methodology (RSM).The quantitative effects of ratio of liquid to raw material, standing time, centrifugationtime were investigated, and a three-level Box-Behnken design which were based onthe results of a single-factor test was employed to optimize the parameters forextraction of cerebroside. The experimental results were fitted with a second-orderpolynomial equation by a multiple regression analysis. By solving the regressionequation and also by analyzing the response surface contour plots, the optimalcerebroside extraction conditions were determined: ratio of liquid to raw material11,standing time60min and centrifugation time8min, and the maximum yield ofcerebroside extraction was2.08%. Residue which was separated from the lipid solublefraction of sika deer antler velvet layer was analysis the functional groups (hydroxyl,amide) by IR. The results show that the residue contains the two functional groupswhich are the characteristics of cerebroside. Thus draw the conclusion: Residue whichwas separated from the lipid soluble fraction of sika deer antler velvet layer wasproved to be cerebroside. Cerebroside has good bioactives of clearing DPPH freeradicals.(2) An antimicrobial cerebroside was extracted using petroleum ether from thelipid soluble fraction of sika deer antler velvet layer which was extracted bysupercritical CO2extraction technology. The effect of different parameters, such as ratio of liquid to raw material, standing time and centrifugation time on the extractionof crude cerebroside is using single-factor experiment. The crude cerebroside waspurified using silica gel column chromatography, was identified using TLC and wascharacterized by employing IR. The biological activities show cerebroside hasobvious inhibitory activities, and the pure one is rather good than the crude one. Theinhibitory activities of them were125μg/mL and27μg/mL on E.coli, respectively. Buthave no bacteriostasis against staphylococcus.(3) In this study the extraction of ganglioside from the lipid soluble fraction ofsika deer antler velvet layer was investigated using response surface methodology(RSM). The quantitative effects of ratio of liquid to raw material, temperature, timewere investigated, and a three-level Box-Behnken design which was based on theresults of a single-factor test was employed to optimize the parameters for extractionof ganglioside. The experimental results were fitted with a second-order polynomialequation by a multiple regression analysis. By solving the regression equation andalso by analyzing the response surface contour plots, the optimal gangliosideextraction conditions were determined: ratio of liquid to raw material13, temperature30℃and time4min, and the maximum yield of ganglioside extraction was1.25%.Residue which was separated from the lipid soluble fraction of sika deer antler velvetlayer was proved to be ganglioside by IR and TLC.(4) In this study the extraction of glycolipids (cerebroside and ganglioside) inbovine spinal cord was investigated using response surface methodology (RSM). Thequantitative effects of field strength, pulse numbers, ratio of liquid to raw material,and a three-level Box-Behnken design which was based on the results of asingle-factor test was employed to optimize the parameters for extraction of glycolipid.By solving the regression equation and also by analyzing the response surface contourplots, the optimal glycolipid extraction conditions were determined: field strength19.7kV/cm, pulse numbers9.1and ratio of liquid to raw material11, and the weightof glycopilid extraction was0.318g per5g bovine spinal cord. And the extraction ofglycolipid by HIPEF is better than other traditional technologies.(5) In this study the extraction of phosphatidylcholine (PC) in sika deer velvetantler slag was investigated using response surface methodology (RSM). Thequantitative effects of field strength, pulse numbers, moisture concent of extractionsolvent, and a three-level Box-Behnken design which was based on the results of asingle-factor test was employed to optimize the parameters for extraction of PC. By solving the regression equation and also by analyzing the response surface contourplots, the optimal PC extraction conditions were determined: field strength20kV/cm,pulse numbers2.48and moisture concent of extraction solvent8.5, and the weight ofPC extraction was1.592mg per1g sika deer velvet antler slag. And the extraction ofPC by HIPEF is better than other methods, because it’s quick and getting more PC. |
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