Mechanism Of Activation Of Oligodendrocyte Precursor Cells By Oscillating Field Stimulation In Spinal Cord Injury Rats

Part ⅠProliferation and differentiation ofoligodendrocyte precursor cells ofspinal cord injury model rats after oscillating field stimulation.Objective: This study is to evaluate the proliferation and differentiation ofoligodendrocyte precursor cells of spinal cord injury model rats after oscillating fieldstimulation. Methods: SD rats were divided into the spinal cord injury group, oscillatingfield stimulation group and the normal group. Oscillating field stimulation group wastreated by oscillating field stimulation. To detect the proliferation and differentiation ofoligodendrocyte precursor cells by means of frozen sections, immunofluorescence stainingand electron microscopy observation after labeled by Brdu and surface markers ofoligodendrocyte lineage cells. Results: The expression level of oligodendrocyte cell line ofoscillating field stimulation group was higher than that of spinal cord injury group in4d,7d,10d and14d, especially in10d and14d. Colocalization expression of NG2-P1ofoscillating field stimulation group was stronger than that in the spinal cord injury group in4d,7d,10d and14d. But in the oscillating electric field group, NG2-P1expression in14dwas weaker than that in4d,7d and10d. Conclusions: Oscillating field stimulation canimprove oligodendrocyte precursor cells proliferation in the injured spinal cord, whichwould contribute to the formation of myelin after spinal cord injury. Part ⅡTheATPand LIF expression level during the activation ofoligodendrocyte precursor cells after spinal cord injury treated by oscillating fieldstimulation.Objective: This study is to evaluate the expression level of theneurotransmitters purine (ATP) and cytokines (LIF)after promoting oligodendrocyteprecursor cells activation by oscillating field stimulation. Methods: SD rats were dividedinto the spinal cord injury group, oscillating field stimulation group and the normal group.Oscillating field stimulation group was treated by oscillating field stimulation. Detectingthe expression levels of ATP and LIF by method of ELISA. Results: The ATP and LIFexpression levels in the spinal cord injury group and oscillating electrical field stimulationgroup were higher than the normal group, there is a statistical difference between thenormal group and the two groups (P<0.05). Within the two groups, ATP and LIF showed agradual increasing trend from the first to the sixth week after spinal cord injury. But ATPand LIF showed a downward trend after the sixth week. Conclusions: Oscillating fieldstimulation can promote ATP and LIF expression levels in the injuried spinal cordespecially early atage after oscillating electric field stimulation. It showed that ATP andLIF played an important role for the activation of oligodendrocyte precursor cells afterspinal cord injury by the treatment of oscillating field stimulation. Part ⅢThe neurological function recovery of spinal cord injury rats treated byoscillating field stimulation.Objective: This study is to assessment the neurological function recoveryof spinal cord injury rats treated by oscillating field stimulation. Methods: SD rats weredivided into the spinal cord injury group, oscillating field stimulation group and the normalgroup. Basso-Beattie-Bresnahan(BBB)score, incline plane test and motor evokedpotential(MEP) were recorded and calculated. Results: The BBB score and incline plane score in the spinal cord injury group were more than that in the oscillating field stimulationgroup within two weeks after spinal cord injury. However, the BBB score and incline planescore in the spinal cord injury group were less than that in the oscillating field stimulationgroup from the third week, there is a statistical difference about the BBB score and inclineplane score between the two groups (P<0.05). The results of MEP in the the oscillatingfield stimulation group was better than that in the spinal cord injury group from first toeighth week. The incidence of ascites is14.7%and25.2%in the spinal cord injury groupand oscillating field stimulation group, there is a statistically significant difference (P <0.05)between the two groups(P<0.05). Conclusions: Oscillating field stimulation caneffectively repair spinal cord injury and promote neurological function recovery. Theinstrument of oscillatingl field stimulation may contribute to the occurrence of ascites inrats affecting the assessment of neurological function in the early stage.