Effects Of Atmospheric-pressure Room-temperature Plasma Jets In The Treatment Of Chronic Periodontitis

Purpose:It has reported that the atmospheric-pressure room-temperature plasma (APRTP) jets have good advantages in inactivating a broad range oforal bacterial biofilms and promoting chronic wounds healing. Since it is a gaseous medium which exists at room temperature, it can penetrate into minor irregular parts to exert its biological effects, and does not cause heat damage. Accordingly, APRTPis an interesting novel biomedical application that is attractive for medical use. In our previous studies, APRTP was effective in killing Porphyromonas gingivalis (Pg) biofilms in vitro while no significant damage to normal osteoblasts. Therefore, the aim of the present study was to evaluate the efficacy and safety of APRTP in the treatment of chronic periodontitis in vivo.Methods:Developed an APRTP source which is suitable for oral use especially for periodontal treatment and make diagnoses of its basic characteristics. Then, the Beagle dog model of chronic periodontitisrapidly establishedcaused by boneless surgery, orthodontic ligatures ligation, tartar filling, and periodontal disease diet. The dogs were randomly assigned to two groups for different treatments:(i) Test group:root surface debridement (RSD) combined with plasma jet irradiation, (ii) Control group:RSD combined with 0.12% CHX rinsing. After treatment, obervastions contain the periodontal parameters (PLI, mSBI, PD, and AL), the detection of periodontal bacterial species and quantity by PCR as well as the changes in alveolar bone height observed on X-ray and CBCT were recorded, and comprehensive analysis of effect of these two therapeutic in the treatment of periodontal disease were made. A preliminary safety evaluation of APRTP was also investigated by stereomicroscope, SEM, FT-IR, and histopathological evaluation.Results:Both of two treatments for Beagle dogs with chronic periodontitis, could significantly improve the clinical parameters, reduce the type and quantity of periodontal bacteria, and promote alveolar bone healing. However, the efficacy of plasma treatment group was significantly better than 0.12% CHX rinsing group; the maintain the efficacy of plasma treatment group was better than 0.12% CHX rinse treatment, too. In addition, no damages were seen on Beagle dog soft and hard periodontal tissues (enamel, cementum, and gingival tissues) after 3min direct exposure to the APRTP.Conclusions:APRTP was an effective and safe adjunctive treatment to RSD for induced chronic periodontitis in Beagle dogs. Thus in the future, APRTP may be could used to obtain the successful periodontal treatment and save more teeth. However, further studies will focus on the biological effects include bacterial biofilms inactivation, tissue healing promotion and biocompatibility of APRTP.