| Part 1 Association between Polymorphism of ADAM33 Gene and Bronchial Asthma in Inner Mongolia Mongolian and Han populationobjective To study the clinical data of bronchial asthma patient of Han and Mongolian population in Inner Mongolia,study T1 T2 V4 S2 genotype and the allele frequency of ADAM33,study the relationship between gene polymorphism and bronchial asthma.Method Use general condition questionnaire and clinical examination to collect general clinical data of bronchial asthma patient,use selected restriction fragment length polymorphism (PCR-RFLP) method to detected ADAM33 gene polymorphism.118 cases of Mongolian and 130 cases of Han patients with asthma were detected, compared with 132 cases of healthy Mongolian and 134 healthy Han respectively as controled and screening significant genes.Results The data of Han and Mongolian population are statistically different in Inner Monglia, including BMI, smokers(%), FEV1%predicted value, FEV1/FVC between bronchial asthma patient and normal person. Three genotypes of T1, T2, V4, S2 sites can be checked out in Mongolian and Han in Inner Mongolia. The bronchial asthma of Han group compared with the control group T1 genotype and allele frequency of AA was statistically significant difference (P<0.05), AG, OR (95%CI) were 2.089 (1.159-3.765),0.449 (0.242-0.831), G allele OR (95%CI) 0.557 (0.329-0.942); comparison was made between the S2 genotype and allele frequency of CC was statistically significant difference (P<0.05), GC, OR (95%CI) were 0.573 (0.349-0.941),1.648 (0.998-1.850), G allele OR (95%CI) 1.559 (1.026-2.371); compared to T2 V4, genotype, allele frequency, the difference was not statistically significant (p>0.05). On the Mongolian bronchial asthma group compared with the control group, T1 genotype and allele frequency of AA was statistically significant difference (P<0.05), AG, OR (95%CI) 2.295 (1.200-4.391),0.395 (0.198-0.789), G allele OR (95%CI) 0.518 (0.291-0.922) to compare; V4 genotype and allele frequency of GC was statistically significant difference (P<0.05), GG, OR (95%CI) were 0.363 (0.179-0.736),2.555 (1.463-4.462), G allele OR (95%CI) 1.715 (1.190-2.473); to compare the S2 site the genotype and allele frequency of GC was statistically significant difference (P<0.05), GG, OR (95%CI) were 0.540 (0.320-0.911),1.802 (1.063-3.055), G allele OR (95%CI) 1.603 (1.028-2.500); the T2 genotype and allele frequency Rates were compared and the differences were not statistically significant (p>0.05). Compare the T2 genotype and allele in Mongolian and Han asthma and control group, there were no significant differences (P>0.05); and T1, S2 genotype and allele in Mongolian and Han asthma and control group were compared, the differences were statistically significant (P<0.05); no significant V4 genotype and allele in Han asthma compared with the control group (P>0.05), but in Mongolian asthmatic patients,the difference was statistically significant.Conclusion The high-risk group of asthma are obese people and smokers, T1, S2 site polymorphism in ADAM33 gene may all play a role in the population which include Han and Mongolian asthma in Inner Mongolia region, and V4 loci polymorphism only play a role in the Mongolian asthma crowd,however T2 loci polymorphism may has nothing to do with asthma, the Han and Mongolian population in Inner Mongolia.Part2 Association between Polymorphism of ADAM33 Gene and Bronchial Asthma in Inner Mongolia Mongolian populationObjective To study the different genotype and allele frequency distribution of ADAM33 gene T1, T2, V4, S2 sites in Mongolian population in Inner Mongolia, to discuss the relationship between ADAM33 gene polymorphism and bronchial asthma.Method selected restriction fragment length polymorphism (PCR-RFLP) method to detected ADAM33 gene polymorphism.180 cases of Mongolian patients with asthma detection, compared with 186 cases of healthy Mongolian as controled and screening significant genes.According to condition,the asthma group was divided into mild, medium and severe groups, including 83 cases of mild group, medium group of 47 cases of 50 cases of severe group, and compared distribution difference of different genotype, and compared every genotype of V4 FEV1, eosinophils, IgE comparison, analysis of the correlation.Results ADAM33 T1 gene AA, AG genotype in the asthma group and control group had significant difference (x22 were 8.810,8.294, P<0.05), OR value was 1.983, 0.500, G allele OR was 0.580; S2 genotype CC, there was significant difference between two groups (x2=4.277, P<0.05), G allele OR was 1.423; V4 loci GC, GG genotype, there was significant difference between two groups (x2 2 were 7.880, 10.313, P<0.05), OR value was 0.459,2.130, G allele OR was 1.496. T2 locus genotypes in asthma group and control group in the distribution of the difference was not statistically significant (x 2,0.248,1.287 and 1.218 respectively, P>0.05). V4 locus genotypes in mild, moderate and severe group frequency distribution was statistically significant (x2 2=16.049, P<0.05), and the genotypes of FEV1 and IgE were compared, the difference was statistically significant (P<0.05), to compare the genotype of eosinophils, there was no statistically significant difference (P>0.05).Conclusion T1, V4, S2 locus polymorphism of AD AM33 gene may play a role in the Mongolian asthma population,However T2 locus polymorphism may has no relationship with Mongolian asthma patients in Inner Mongolia. V4 gene polymorphism may be related to the severity of asthma, and can be combined with pulmonary function and IgE, as a potential indicator to assess the severity of bronchial asthma. |
