Experimental Study Of Effects Of Ferulic Acid On Human Osteosarcoma Cell Lines

part one:Ferulic Acid Promoting Apoptosis in Human Osteosarcoma Cell LinesObjective:The purposes of this study were to explore the promoting apoptosis and antitumor activities of ferulic acid(FA)in human osteosarcoma and its potential mechanisms.Methods:The SaOS-2 and MG63 osteosarcoma cell lines were opted to experiments and these cells were,respectively,cultured with various concentrations of FA(0 ?M,10 ?M,20?M,40 ?M)for 72 hours at 37?.The viabilities of the FA treated cells were monitored by MTT.Apoptosis cells were evaluated using annexin V/PI by flow cytometry.Apoptosis proteins caspase-3,procaspase-3,Bcl-2 and Bax were detected by western blot.Expressions of apoptotic genes Bcl-2 and Bax were quantified by qPCR.Results:The cell viabilities were critically declined in the concentration-dependent manner in FA groups(P<0.01).The apoptosis cells were increased proportionately with the concentration of FA(P<0.05).The procaspase-3 protein contents,and Bcl-2 mRNA and protein contents were significantly decreased while caspase-3 protein contents,and Bax mRNA and protein contents were concomitantly increased in the concentration-dependent manner in FA groups(P<0.05).The response to FA by the SaOS-2 osteosarcoma cell was similar with the MG63 osteosarcoma cell(P>0.05).Conclusion:FA could significantly descend osteosarcoma cell viability through the promoting apoptosis pathway in which FA activates both caspase-3 and Bax and inactivates Bcl-2.part two:Ferulic Acid Inhibiting Angiogenesis in Human Osteosarcoma Cell LineObjective:To explore angiogenesis in osteosarcoma cell line cultured by ferulic acid.Methods:The SaOS osteosarcoma cell had,respectively,been cultured in 40 ?M or 0?M ferulic acid solution for 72 h.Vascular endothelial growth factor(VEGF)protein were detected using immunohistochemistry.The relative expression of VEGF mRNA were quantified by qPCR.VEGF protein contents were observed via western blot.Then the cultured SaOS-2 cells were inoculated in Balb/C nude mice,and transplantation tumor were checked via HE staining,VEGF immunohistochemistry,CD34 immunohistochemistry,and calculation of microvascular density(MVD).Results:In vitro,VEGF immunohistochemistry,the relative expression of VEGF mRNA and VEGF protein contents in ferulic acid group were obviously decreased.In vivo,morphological observation of transplantation tumor illustrated that heteromorphism was not apparent in the cells of ferulic acid group and vascular systems were scanty in its tumor tissure.Indeed,immunohistochemistry of transplantation tumor revealed that both VEGF stains and CD34 stains were significantly reduced in ferulic acid group,and MVD in ferulic acid group(7.70�1.55)were obviously less than that in control group(18.22�3.31)(P<0.05).Conclusion:angiogenesis in osteosarcoma could be inhibited by ferulic acid,which is expected to provide a novel and attractive target of therapeutic strategies of osteosarcoma.