Expression Of CD105,CD34 And VEGF In NSCLC And Their Significance

Backgrand and Objective: Angiogenosis of tumor is the pathological basis which the growth and matastasis of cancer depend on, because it not only provides plenty of nutrient to carcinorina but also coutinuouly exports tumor cells to neighboring tissues and organs or to the distants.therefore, the studies of rumor angiogenosis are helpful to master the mechanisms of tumor growth, in the same time ,it is useful for tumor clinical diagnosis and analysis of tumor prognosis,how to block tumor angiogenosis is providing a promising therapeutic perspective, the present, pan-endothelial markers, such as CD-64,are generally used in evaluation of angiogenesis, pan-endothelial antibodies react with not only "newly forming" vessels but also normal vessels just trapped within tumor tissues.it has been recently reported by foreign article that anti-CD105 antibody preferently reacts with "activated"endothelial cell in angiegenic tissues.moreover, increasing evidence suggests that CD105 is a new powerful marker of neovascularization in solid malignancies.so the purpose of this experiment is to explore popularizing CD105 as a kind of basic marker of a active vascular endothelium by comparing the expression of CD105 and pan-vascular endothelial marker CD34 in NSCLC, as well as the correlation among CD105,CD34 , VEGF.Vascular endothelial growth factor(VEGF),also known as vascular permeability factor(VPF), one of the most potent angiogenic molecules, regulates both angiogenesis and vascular permeability, and hence promotes tumor progression and development of malignant pleuraleffuse ions in NSCLCo Signals via epidermal growth factor receptor (EGFR) promote not only the tumor cell cycle, but also the process of angiogenesis These molecules are potentiall targets for anti-tumor vascularture therapy.. Many agents targeting tumor vascularture have been developed, and several compounds have shown anti-rumor potential As a relative mature marker, here it have been further studied that VEGF express in NSCLC and relation between VEGF and CD105/CD34Methods :40 cases of carcinoma specimens were collected with 20 cases of lung tissue beside cancer regarded as the control group, the S-P immunohistochmical technique was used to detect the expression of CD105 CD34 and VEGF in both the experimental and control group.SPSS10.0 was used to evaluate the statistical significance.Results: 1. The MVD value of the 40 cases lung carcinoma marked by CD105 and CD34 were 29. 82 + 9.29/400 X (scope) and 36.66 + 10.11/400 X (scope) respectively,both were significantly higher than those of control tissue which were 9.57 + 7.23/400 X (scope) and 27.35 + 10.42/400 X (scope),(p < 0.01).both was signicantly correlated with the tumor volume; furthermore CD105 was correlated with the lymph-node metastasis histoiogic type.and tumor differentiation (p<0.05).2. the rate expression of VEGF was 65%(26/40);higher than control group 30%(6/20).there was a significant correlation between VEGF and CD105(CD34),it seems that CD105 was more closely correlated with VEGF (r1=0.803, r2=0.513).: r1 >r2.Conclusion: 1. Both of CD105 and CD34 are important marker of the endothelial cell of vessels.2. CD105 was more sensitive and specific than CD34 for staining endothelial cells when tumor's distant metastasis and histological grade etc were assessed.3.There are significant correlation among CD105. CD34 and VEGF with each other: CD105 was more closely associated with VEGF than CD34.4.The expression of VEGF in NSCLC apparently is higher than it dose in normal issue, the close-relation between VEGF and N'SCLC has been Droved.5. Anti-CD105mAb proved to be superior to anti-CD34mAb in evaluation of angiogenesis in NSCLC and is fit to be taken as a formal active vascular endothelia marker in this tissue.