Polysaccharide Of Angelica Sinensis Protects Rat Chondrocytes From H₂O₂-induced Apoptosis Through Its Antioxidant Effects In Vitro

Objective:Toexplore the protective effects of angelica sinensis polysaccharide?ASP?on rat chondrocyte injury induced by hydrogen peroxide?H₂O₂?.Methods:Rat chondrocytes were extracted and cultured as well as treated with different concentrations of ASP alone or in combination with H₂O₂,and they were measured with cell viability,apoptosis,release of inflammatory cytokines,such as interleukin-1 beta?IL-10?and tumor necrosis factor-alpha?TNF-a?,activities of superoxide dismutase?SOD?and catalase?CAT?,and levels of malondialdehyde?MDA?production,respectively.In addition,quantitative real-time reverse transcription polymerase chain reaction was used to measure the relative expression levels of osteoarthritis?OA?-associated genes,such as type ?collagen?Col2a1?,aggrecan,SOX9,matrix metalloproteinase?MMP?-1,-3,and-9,as well as tissue inhibitor of matrix metalloproteinase?TIMP?-1,respectively.Results:ASPprotectedrat chondrocytes from H₂O₂-induced oxidative stress and subsequent cell injury through its antioxidant,antiapoptotic and anti-inflammatory effects in vitro.Conclusions:Ourstudy suggests that ASP could become a therapeutic supplementation for the treatment of OA.