Study On The Feasibility Of Angelica Sinensis Polysaccharide As Magnetic Nano-material Carrier

This paper mainly studies on the feasibility of ASP as nano- material carrier. This paper summarizes the preparation method of ASP- magnetic nanoparticles, the optimization of preparation technology, the evaluation in vivo and vitro, and the study on the performance of carry drugs. Finally, from the results of above study, the feasibility of ASP as carrier overall can be evaluated.Partâ… The preparation of ASP- magnetic nanoparticlesStudy on the preparation of ASP- magnetic nanoparticles, and determine the best technology by orthogonal test. Appraise the feasibility of ASP as nano- material carrier. Prepare three products respectively by one step reaction, two step reaction and synchronous curing-reaction. And then compare different products'characteristics to determine the preliminary preparation technology. To determine the best technology, the influences of the gram-molecule concentration of Fe²⁺ (mol·L^-1), the ratio of the gram-molecule concentration of Fe²⁺ and Fe³⁺, the volume of NaOH(mL) and the temperature of the reaction(℃) were studied and the orthogonal experiment of four-factors-three-levels was carried out by orthogonal test. Then test and verify the best technology. The results showed that both the magnetic force and stability of the products prepared by synchronous curing-reaction technology are better than that of the products prepared by the other two kinds of technology, and synchronous curing-reaction method is the best technology to prepare magnetic nanoparticles. The best technology is the gram-molecule concentration of Fe²⁺ is 0.30 mol·L^-1, the ratio of the gram-molecule concentration of Fe²⁺ and Fe³⁺ is 1:1, the volume of NaOH is 8 mL and the temperature of the reaction is 80℃. ASP can be used as magnetic nano- material carrier. Partâ…¡The vitro evaluate of ASP- magnetic nanoparticlesStudy on the physical and chemistry characters of ASP- magnetic nanoparticles, and then evaluate the quality of products. To determine the preliminary purified method through the comparison of the products before and after purifying. To determine the particle size, shape, appearance and magnetism through the methods such as Fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), transmission electron microscopy (TEM) and Vibrating Sample Magnetometer (VSM). According to the"Chinese Pharmacopoeia"2005 edition and requirements of materials drug, the examination method of relative components in the material was established. To test content of the mainly materials and study the stability of the products. The results showed that the surface of Fe3O4 is successfully covered with ASP, and the particle size of the individual nanoparticles is about 10 nm. The quality of the product is consistent with the"Chinese Pharmacopoeia", and the content of the product is stabilized. The content of Fe3O4 is about 47% and the polysaccharide content is about 17%. The magnetism and stability of the product are excellent. The characters of the ASP magnetic nanoparticles are stabilization and the quality of it is excellent. To appraise the feasibility from the vitro evaluation, ASP can be used as magnetic nano- material carrier.Partâ…¢The vivo evaluate of ASP- magnetic nanoparticlesStudy on the pharmacokinetics and the tissues distribution of ASP- magnetic nanoparticles in rats. Do the physiological disposition research in rats, and evaluate the feasibility of the ASP as carrier. Take the serum iron of SD rats as measurement indicators, and measure it by atomic absorption spectrophotometry. The pharmacokinetic parameters were calculated with DAS2.0 software package. Inject the ASP- magnetic nanoparticles through caudal vein, and test the Fe content of the individual tissue, and then determine the tissues distribution of nanoparticles in rats. The results showed that the pharmacokinetic process of ASP- magnetic nanoparticles in rats is corresponding with two compartment models. The ASP- magnetic nanoparticles has the nature liver targeting and good magnetic targeting. From the result of vivo evaluation, we can know that ASP can be used as magnetic nano- material carrier.Partâ…£Study on the drug loading property of ASP- magnetic nanoparticlesUse 5-Fu as model drug, and use the glutaraldehyde as cross linking agent, synthesize the ASP 5-Fu magnetic nanopartiles by the method used in ASP-magnetic nanoparticles preparation. Evaluate the feasibility of the ASP as carrier by using the vitro evaluation of ASP 5-Fu magnetic nanopartiles. Determine the particle size and appearance of the product by TEM. And obtain the magnetism property by the sedimentation method. Achieve the drug-loading rate and entrapment rate by the in vitro dissolution test. The results showed that the particle size of individual nanoparticles is about 10 nm. The drug-loading rate of the product is about 18.87% and the entrapment rate is about 28.65%, higher than the results reported in the document. The cumulation drug release in 30 h reaches 77.25%, and the product has the controlled release ability. According to study on the drug loading property of ASP- magnetic nanoparticles, we can say that ASP can be used as magnetic nano- material carrier.