Study On The Construction Of Functionalized β-TCP Porous Scaffolds

The bone defect caused by inflammation,trauma and tumor is a common disease in clinic.The clinical application of the “gold standard” bone autograft is limited due to the limitation of source and immune rejection and the risk of disease.With the continuous development of material technology and biotechnology,the emergence of bone tissue engineering has put forward a new idea to solve this problem completely.This article in biocompatible and biodegradable β-TCP porous scaffolds based on by bionic and biological engineering technology and methods of the scaffold was modified,improve scaffold for cell identification and biological activity,promote the regeneration and repair of bone tissue.1)There are good biocompatibility and biological safety of 3D printing β-TCP scaffolds in ex vivo and in vivo.3D printing β-TCP scaffolds were favor for mBMSC cell adhesion and proliferation without toxic in ex-vivo,and were cause not major organ toxicity in vivo.3D printing β-TCP scaffolds has a good physical properties and biological safety of bone repair scaffold.2)3D printing β-TCP scaffolds coated with collagen I that functional modification,improve the biocompatibility of the material surface,it is conducive mBMSC cell adhesion,proliferation and osteogenic differentiation.Type I collagen coating print 3D β-TCP scaffolds recombinant mineralized surface,promoting the early mineralization mBMSC cells,strenbbgthen the response effect mBMSC cells and 3D printing β-TCP scaffolds between.3)3D printing β-TCP scaffolds coated with collagen I and growth factor PIGF complex functional modification.PIGF growth factor work on mBMSC cell is a multifunctional growth factor,mBMSC can promote cell adhesion,growth,and differentiation into osteoblasts and secrete vascular-related growth factors.Type I collagen and growth factor PIGF compound and dried in different conditions can affect mBMSC biological properties which seeded in 3D printing β-TCP scaffolds..4)3D printing β-TCP scaffolds coated with collagen I and transgenic pigf-tk-CHO cell complex functional modification,to achieve the 3D print β-TCP scaffolds can regulate the expression of growth factors.The system is based on the regulation of gene expression induced by tetracycline Tet-on and ganciclovir induction of apoptosis HSV-tk / GCV suicide gene."Double safety system" to improve the safety and effectiveness of biological gene therapy of bone repair,it could be achieved ex vivo growth regulator,as an ideal way to functional tissue engineering scaffolds.