New Alveolar Bone Scaffold Composite Materials To Repair Bone Defect

Objective: To investigate the repair ability of porous polyacrylonitrile-based carbonfiber/chitosan/biphasic calcium phosphate/poly lactic acid-glycolic acid (PAN/CS/BCP/PLGA)composite scaffold transplanted onto the alveolar bone defect of rabbit.Methods: To use wet synthesis method to prepare Biphasic calcium phosphate andvacuum freeze-drying technology to prepare porous PAN/CS/BCP/PLGA compositescaffold. Measure the compressive strength, porosity by Archimedes method and invitrobiological activity. And use XRD, SEM for analysis of phase structure and morphology.Select32adult male New Zealand big white rabbit, divided into the experimental groupand the control group, alveolar bone defeat area of10mmx10mmx3mm prepared. Theexperimental group is transplanted with PAN/CS/BCP/PLGA composite scaffold and thecontrol group is transplanted with CS/PLGA, followed by observation in2,4,8, and12weeks,respectively, for radiologic analysis and under scanning electron microscope (SEM) andosteoblast count analysis.Results:12weeks after the test results, radiographic evaluation showed the resistance feltat the bone defect area is similar to auto-grafting bone. Histologic observation showed that thebone defect area is completely filled with new bone, and is more mature shelf bone, with auto-grafting bone fully merged. The SEM results show bone formation at bone-material interface,which is replaced with mature bone tissue. Analysis of osteoblast count: active growth ofosteoblast with statistically significant difference.After adding4wt%PAN to PAN/CS/BCP/PLGA composite scaffold, the averagerate is more than85%porosity, the compressive strength increased6-fold is greater than5.9MPa, and the biodegradable rate is lower than that of9.5%, It presents the porouspenetration appearance, the average pore diameter is400-500μm, PAN is well distributed.In vitro modeling the surface of scaffolds produce bone-like apatite.Conclusion: The porous PAN/CS/BCP/PLGA composite scaffold have good biologicalactivity and biodegradable nature, and is better than CS/PLGA composite materials forrepairing bone defects, providing a theoretical basis for using alternative materials as bonetissue engineering scaffolds.