The Role And Mechanism Of Retinoic Acid Signaling Pathway In Wound Repair During The Wound-healing Process After Thulium Laser Resection Of The Prostate

BACKGROUND: The wound-healing process is very important for reducing complications after thulium laser resection of the prostate(Tm LRP).The retinoic acid(RA)signaling pathway has been well studied in the wound-healing process of the skin and other organs.The goals of this study were to identify the role of RA signaling in the repair of the prostate after TmLRP and to investigate the molecular mechanism of this process.METHODS: The expression of retinoic acid receptors(RARs)was analyzed in vivo by immunohistochemistry(IHC),real time qPCR,and western blot analysis.THP-1 cells were co-treated with or without RA and stimulating factor and then assessed by ELISA and qPCR.The supernatants from these cells were cultured with stromal cells and vascular endothelial cells,and the effects on these cells were analyzed.RESULTS: RARs were present in the prostate,and their expression was increased after TmLRP.RAR? was expressed in the macrophages and may be related to the role of stromal cells in the wound-healing process.In vitro,RA enhanced the function of antiinflammatory macrophages and promoted stromal cell activation and angiogenesis.Arg1 was also increased via RAR? after treatment with RA.CONCLUSION: We found that RA signaling was involved in the wound-healing process of the prostate after TmLRP.RA treated macrophages activated stromal cells and promoted angiogenesis.RAR? was the key isoform in this process.