Effects Of Nanomaterials On The Structure And Assembly Characteristics Of Actin

In recent years,nanomaterials have a huge difference in performance from the common microscale materials because of their structure's intrinsic properties and their unique small size effects.This difference also brings infinite potential to nanomaterials.These special properties have made nanomaterials successfully applied in the fields of micro and nanoscale electrons and semiconductors,and are now becoming more and more widely used in biomedicine and have been paid more and more attention.Because of its special size and structure,nanomaterials bring extraordinary surface / interface effects,quantum tunneling effect and small size effect.In practical applications,nanomaterials have the advantages of large specific surface area,many active sites,high reactive activity,strong adsorption capacity,high catalytic activity and low toxicity.It also laid a solid foundation for the application of carbon nanomaterials in biomedicine.But at the same time,through more and more research reports,it is not difficult to find that the nanomaterials,which have great potential for application,are gradually showing the other side of a double-edged sword in the field of biology.When these exogenous nanomaterials enter complex organisms,can their unique properties continue to appear? Or will it lead to some bad biological effects? Because of its huge specific surface area and good adsorption property,it can adsorb some proteins and enzymes in the body after entering into the organism,thus forming a protein halo.This adsorption will alter the normal electrical properties,structure and physiological functions of protein to some extent.Under certain conditions,nanomaterials interacting with proteins will also be affected.This means that the distribution,toxicity and biological effects of specific carbon nanomaterials are uncertain after entering into the biological system.The study of the interaction of nanomaterials and proteins in the field of biomedicine has become the basic problem that these new materials can be used safely in the field of biomedicine.In general,carbon nanomaterials are the first to pass through the cytoskeleton.In this study,we chose the main components of the cytoskeleton,monosomatic actin and actin,to study the interaction mechanism and influence of several common nanomaterials with the two.1.The interaction between two affinity gold nanoparticles and monomer monomer actin was discussed by means of optical absorption spectroscopy and fluorescence spectroscopy.We found that two amphiphilic gold nanoparticles and actin mono spontaneously formed a nanoparticle protein complex,and we have studied the binding constants of the two and the information of the thermodynamic parameters such as Gibbs free energy through the quenching method of fluorescence spectrum,and through further analysis,we initially think that two The intrinsic fluorescence of the actin monomers is seriously quenched by amphiphilic gold nanoparticles,and the adsorption is spontaneous,and the adsorption is very reliable,which is a relatively stable system.And two Pro gold nanoparticles do not change the two stage structure of actin monomers.These experiments results that our homemade two Pro gold nanoparticles will have a certain effect on actin monomer,but the simple adsorption of both of them does not change the structure of the protein,and the gold nanoparticles are also proved to a certain extent.The security of the application of particles in this system,and through further in-depth study in the future,we may be able to make use of this system to produce some stable,readily available advanced nanostructures.2.Carbon nanomaterials,because of their excellent adsorption capacity and large specific surface area,are currently being applied more and more widely in biological drug delivery and disease treatment.At present,some studies have shown that carbon nanomaterials can inhibit the proliferation and movement of certain cancer cells through the activity of actin in the cytoskeleton,in addition to carrying anticancer drugs.Therefore,we studied the effects of carbon nanomaterials(graphene oxide and graphene quantum dots)on actin in molecular level.By means of circular two chromatography,scanning confocal microscopy and atomic force microscopy,we found that graphene oxide could change the two and three structure of actin monomer and further affect its ability to polymerize into actin.The above results can explain the influence of carbon nanomaterials on cytoskeleton at molecular level.