To Study The Fresh Mare's Milk Powder Preparation Technology And Detection Its Nutrients And Efficacy

Fresh mare's milk is a kind of deep cultural heritage,distinctive regional ethnic characteristics of grassland resources.Contains protein,unsaturated fatty acids and trace elements,with tonic physical,skin dryness,heat and thirst effect,ordinary people eat,physical health,anti-aging.It has anti-fatigue,anti-oxidant,immune regulation,treatment of tuberculosis and other effects.These proteins involved in human metabolism,regulate physiological functions,improve human immunity and prevent disease,unsaturated fatty acids and low molecular weight fatty acids in the prevention of hypercholesterolemia,atherosclerosis have a good effect.There are a lot of horse products abroad,while domestic acid mare`s milk only.Therefore,in this study the following four aspects of fresh mare study reveal its special proteins of components to verify its unique nutrition and health claims,and further determine its therapeutic value,safety features provide milk market,stable,reliable The new product has a health care function,the application of modern techniques to reveal fresh mare traditional medical value,provide a solid foundation for the functional orientation experiments fresh mare`s milk:?1?were used to freeze-drying and spray drying fresh horse milk,determine its process parameters;?2?fresh mare with milk,camel milk,donkey milk basic components were compared,and the fresh mare's milk proteins were analyzed;?3?fresh horse milk anti-fatigue,immune regulation and anti-function of oxidation,and other health care;?4?A preliminary study of the separation of fresh mare Manglietia antioxidant activity peptide purification and anti-oxidative activity.Provide theoretical and experimental basis for the above study by fresh mare functional orientation.1 Preparation of fresh mare`s milk powder of freeze-drying and spray drying processThis study was prepared by two fresh mare`s milk,screened process parameters:?1?Spray drying process parameters screening:the fresh mare`s milk powder flowability,bulk density,moisture content,flour yield,protein content as an indicator of the proportion of concentrated study different feed flow,affecting the outlet temperature of the spray drying effect.Using L<sub>9</sub>?3<sup>4</sup>?orthogonal experimental design to explore the preparation of fresh mare`s milk spray drying process parameters.The results for the concentration ratio of 45%,the feed flow rate of 400ml/h,outlet temperature is 80?,the fresh mare`s milk are in line with the provisions of the various indicators,provide a reference for the production??2?Freeze-drying process parameters Filter:under vacuum conditions to concentrate ratio of fresh mare's milk,thickness,sublimation drying time as the study factors to fresh mare`s milk powder flowability,bulk density,moisture content,flour yield,protein content as an index using L_9??3⁴orthogonal experimental design to explore the preparation of fresh mare`s milk freeze drying process parameters.The results for the fresh mare`s milk best vacuum freeze-drying process parameters for fresh mare thickness0.5cm,32.0 sublimation drying time,the proportion of 45%concentrate,can provide a reference for the production.?3?Compared the Spray drying process and Freeze-drying process parameters,Considering the fresh mare`s milk freeze-drying and spray drying of the advantages and disadvantages,because the spray drying spray freeze drying faster than the speed,low energy consumption,consuming short,convenient and suitable for large-scale production conditions,so make sure the spray drying fresh mare`s milk is the best preparation processes2 Mare's milk powder,fresh mare`s milk?cow milk,camel milk,donkey milk composition analysis and comparison of the basic components and fresh mare's milk proteinThis study firstly,fresh milk,fresh milk and fresh milk,fresh donkey milk flavor,appearance,pH,acidity,density,total protein,total fat,17 kinds of amino acids,9unsaturated fatty acids,calcium,iron,zinc was detected and compared;results show that,the calcium content in fresh milk 74.75mg/100g,iron content is 0.055 mg/100g,the zinc content was 2.4mg/100g,the phosphorus content was 30.6mg/100g,the order of fresh milk,fresh milk,fresh milk and fresh donkey milk;fresh mare's milk in 9 kinds of unsaturated fatty acid determination results show that,the human body can not be synthesized,infant brain development must linoleic acid,linolenic acid content,linolenic acid was detected only in the fresh mare's milk for 12.4%,the rest of the fresh milk,not fresh donkey milk,fresh milk;17 kinds of amino acids in order of fresh milk and fresh milk,fresh milk and fresh donkey milk.Fresh mare's milk powder and fresh milk were compared.Results show that fresh mare's milk powder in loss of spray drying process of some trace elements and fatty acids,and fresh milk were significant differences compared with statistical significance,but the rest of the component change is not obvious.by SDS-PAGE and QE-LC-MS method for analysis of fresh mare's milk proteins,and protein profiles and compare the library to seek fresh mare's milk protein composition,by GO method to detect molecular function of its protein,respectively?molecular function?,biological processes?biological process?,cells?cell component?are classified in three ways,revealing fresh mare biological functions.The results showed that fresh mare's milk powder have the rich nutritional.Fresh mare proteomics results show that 283 kinds of fresh mare's milk contains proteins,these are involved in cell proliferation?positive and negative regulation apoptosis?oflactose synthetase activity?positive and negative regulation of cell growth?the negative regulation of angiogenesis?immune response?tumor markers,further reveal the physiological activity of fresh mare's milk.3 Study the fatigue,immunomodulatory and anti-oxidation effect of fresh mare`s milkIn this study,fresh mare`s milk health functions studied by the following three aspects:1)anti-fatigue effect of fresh horse milk Study:fresh mare`s milk oral administration of 45 days,were forced to swim,climb poles,serum urobilin determination determination of blood lactate,blood lactate dehydrogenase activity assay and glycogen content was measured.The results show that the high dose group of fresh mare`s milk can significantly improve the glycogen content and serum lactate dehydrogenase activity,prolong swimming and climbing pole time,and significantly lower serum urea nitrogen and lactic acid content,and compared with the control group were significantly difference.Description fresh mare`s milk has a certain degree of anti-fatigue effect;2)fresh mare`s milk in vivo antioxidant research:study of fresh mare`s milk for D-galactose-induced oxidative injury in mice in vivo antioxidant effect.Gavage 45 days,measured in mouse liver,spleen and serum xanthine oxidase?XOD?,myeloperoxidase?MPO?,protein?Coomassie blue staining?,nitric oxide synthase?NOS?,propionate dialdehyde?MDA?,glutathione?GSH?,nitric oxide?NO?,superoxide dismutase?SOD?content and body weight changes were observed in mice trend.Compared with the model group,fresh mare`s milk can antagonize mice weight loss,enhanced serum,liver and spleen activity of SOD and GSH reduce MDA,MPO,XOD,NO,NOS content.So fresh mare`s milk has a role to prevent oxidative damage,which may be related to inhibition of free radicals,increase the activity of antioxidant enzymes in the body,improve the body's antioxidant capacity.3)Fresh mare`s milk immunomodulatory effects research:by observing the index of immune organs of mice,carbon clearance capacity,delayed-type hypersensitivity,serum hemolysin level of splenocytes value-added,cytokine secretion,index of T-lymphocyte subsets,such as fresh mare`s milk on immune function in mice.Compared with the control group,the fresh mare`s milk can promote the development of thymus and spleen,and delayed type hypersensitivity occurs,increase the ability of mouse carbon clearance and serum hemolysin level,promote spleen lymphocyte proliferation and cytokine secretion,promote T secretion of lymphocyte subsets.So fresh mare`s milk have significant immune enhancement?4 Separation?purification the antioxidant peptide from Fresh mare`s milk and and test it`s antioxidant activityTo study in three steps:1)Content of peptides?the ability to restore hydrolysates,and hydroxyl radical scavenging,superoxide anion scavenging,DPPH scavenging antioxidant activity and other indicators to the type of enzymes?trypsin,stomach protease,papain,chymotrypsin?,reaction temperature?25,35,45,55,65??,hydrolysis time?1,2,3,4,5h?,enzymatic PH value?5,6,7 8,9?,and the enzyme substrate ratio?30:1,40:1,50:1,60:1,70:1?to investigate the factors,screening for trypsin digestion process parameters,enzymatic 3.41h,reaction temperature is 50.14?,hydrolysis PH of 8.0,and the enzyme substrate ratio of 59.19:1,in this condition hydrolysates highest antioxidant activity;2)Respectively using 3kd and 10kd,30 kd molecular weight of ultrafiltration membrane of the enzyme solution ultrafiltration,and<3kd,3¹0kd,10³0kd,>30kd 4kinds of molecular weight range of enzymatic hydrolysis,respectively,measure its Content of peptides,reducing power,hydroxyl radical scavenging ability,ultra oxygen anion removal ability,DPPH removal ability,screening of antioxidant ability strong part.Results for the range of 3¹0kd molecular weight enzymolysis relatively strong antioxidant activity,the freeze-dried standby for further separation.