Design,Synthesis And Property Study Of Smart Nanoprobe In Tumor Theranostics

In recent years,nanomaterials are widely applied in the integration of tumor theranostics due to their unique physical and chemical properties.Based on the controllable design of functionalized nanomaterials,four new smart nanoprobes for tumor theranostics were prepared,which provide a new method for the diagnosis and treatment of tumor.This content can be divided into the following four parts:(1)Cu O-Ni O/C heterojunction nanofibers were prepared through controllable electrospinning and thermal treatment.Under visible light irradiation,the resultant Cu O-Ni O/C nanofibers may catalyze decomposition of AB to H2,generating pressure as a readout means.This pressure-responsive nanosensor has a great potential for cancer cells detection by portable pressure meter.In addition,the resultant Cu O-Ni O/C nanofibers may also catalyze reduction of Rh6 G,resulting in fluorescent signal change as another readout means,which could further “see” the specific cancer cells by fluorescence imaging means.Moreover,such dimode platform could sensitively determinate Hela cells from artificial whole blood samples.(2)Monodisperse and concave spherical Fe3O4@Cu2O NCs with high photothermal conversion efficiency,high SERS activity and good MR property for MRI-SERS imaging-guided photothermal therapy(PTT),which were prepared by a novel and facile method for one-step synthesis method.The reaction time and the amount of Se powder play an important role in formation of concave spherical Fe3O4@Cu2O NCs.The formed concave structure provides the light harvesting and efficient adsorption of probe molecules.Consequently,the resultant concave spherical Fe3O4@Cu2O NCs exhibit ultrahigh SERS activity(EF up to 6.62 ×105)toward R123 molecules with detection limit of 3×10-8 M.Benefit from high SERS activity of Fe3O4@Cu2O NCs to R123 molecules,the concave spherical Fe3O4@Cu2O-PEG-FA NCs is successfully used to specifically recognize and image Hela cells.In addition,the resultant Fe3O4@Cu2O NCs also enable targeted T2-weighted MR imaging of Hela cells.Furthermore,due to its high photothermal conversion efficiency,the effective destruction of He La cells was successfully realized at a very low irradiation power density.(3)A facile seed-mediated growth method was used to synthesize sub-30 nm Au3 Cu TPNCs,and effectiveness of these TPNCs as multimodality imaging-guided NIR-II agents has been evaluated.Owing to its maximum absorption peak completely located in the NIR-II region,the Au3 Cu TPNCs exhibited good photostability,deep tissue photothermal therapy,and an excellent NIR-II photothermal performance with a large extinction coefficient of 53.0 Lg-1 cm-1 and a significantly high photothermal conversion efficiency of 75.27%,which is the highest value among reported inorganic photothermal agents with small size to date.Modification with PEG and FA significantly improved their biocompatibility and tumor targeting.The obtained Au3Cu@PEG-Cy5,FA NCs not only could selectively and quickly eradicated tumors in NIR-II biowindows without side effects in tumor-bearing mice but also show excellent MOST,NIR fluorescence and NIR thermal imaging potential.This study opens up a novel approach to develop sub-30 nm gold-copper bimetallic nanostructure as a powerful platform for use in multimodal image-guided photothermal therapy with significant therapeutic effect in NIR-II biowindows.(4)Firstly,Fe/Fe3O4 nanocrystals were synthesized by a simple thermal decomposition method.Subsequently,a variety of polymer systems were constructed by the organic functionalization process: PLGA-PEG,PLGA-PEG-FA and PLGA-PNIPAM;finally,the functional nanospherers of ICG@Fe/Fe3O4@PLGA-PEG-PNIPAM-FA were preparedby Oil/Water(O/W)solvent evaporation method with the ultrasonic instrument system.The fluorescence imaging and magnetic resonance imaging of the nanospherers in vivo demonstrated the potential applications of multimodal imaging.In addition,it was easy to catalyze the production of reactive oxygen species(ROS)in the water,which provides the basis for the treatment of ROS in the tumor.Furthermore,under the irradiation of laser,local heat could be produced inside the microsphere,which triggers the change of the polymer configuration of the microspheres.Through the drug loading experiment,we proved the controllability of the drug release process by p H and laser.A consistent result was also found in the killing process of tumor cells.Finally,multi-mode imaging mediated ROS therapy and controlled drug delivery system were realized.