Detection Of T-2 Toxin Based On Nanomaterials And Biofilm Interference Technology

T-2 toxin is one of the most toxic trichothecenes A,which produced by fungi such as Fusarium oxysporum and Fusarium oxysporum.The toxin mainly contaminates crops such as barley,wheat,corn,and can cause harm to animals and humans who ingested the contaminated crops.In addition,T-2 toxins may be retained in the edible organs of the domestic animals in the form of toxic metabolites after being ingested by domestic animals,which endangered human health at the same time.It can cause skin-irritation to humans through contacting skin.T-2 with a high-dose can also cause harm to the functions of immune system,digestive system and nervous system in human.And due to the stable chemical structure,the heat resistance,acid resistance and some other properties of T-2 are outstanding,which means,the toxicity will not decrease in six to seven years in the daily environment.So,T-2 poses a great threat to human health.Therefore,how to detect T-2 toxins in crop products quickly,accurately and at low cost is very important.In this paper,three methods for detemination of T-2 toxins in crops have been developed by applying fluorescent quantum dots,magnetic microspheres and biolayer interferometry.The main research contents of this paper are as follows:1 Synthesized and characterized the Fe₃O₄ nanoparticle-embedded magnetic polystyrene nanospheres,and modified the surface of the microspheres with specific antibodies to construct a magnetic probe specific to T-2 toxin.The fluorescent probe was prepared by coupling T-2-BSA to the surface of fluorescent quantum dots.In detection,the fluorescent probe and the T-2 toxin that may be present in the sample to be detected are competitively combined with the magnetic probe to generate different fluorescent signals for rapid and accurate detection of the T-2 toxin.This method has made full use of the magnetic properties of nanospheres and the high fluorescence intensity of the QDs,and achieves the effects of rapid separation and trace detection.2 Based on the method above,the T-2 toxin-specific antibody is coated on the surface of a 96-microwell plate through hydrophobic interaction to prepare a microwell plate that can specifically bind to the T-2 toxin.Then QDs compete with T-2 toxins for binding specific antibodies on microplates which generate fluorescent signals of different intensities as the result.Compared with the method above,this method reduces the space for reaction by placing the reaction in a 96-microwell plate,and achieves high-throughput detection without compromising accuracy,while reduce the time to completed and the consumption in reagent,which makes the determination cost-effective.3 Biolayer interferometry?BLI?is an optical analytical technology for real-time determination of interactions between biological molecules.The toxin-specific antibody and the toxin in sample are quickly connected to the sensor during detection by modified the APS sensor with T-2-BSA.And then the analyzer can measure changes in thickness on the sensor layer,which is related to the content of T-2 toxin.This method can visualize the real-time immune reaction in the detection,and is fast,simple-operation,and easy monitoring with high throughput.